MIG Mouse

CXCL9 was initially identified as a lymphokine-activated gene in mouse macrophages . The human version of CXCL9 was later cloned using mouse CXCL9 cDNA as a probe . The gene is induced in macrophages and primary glial cells in response to interferon-gamma (IFN-γ) stimulation .

CXCL9/MIG plays a crucial role in the immune response by acting as a chemoattractant for activated T cells . It is predominantly expressed by monocytes, macrophages, hepatocytes, and endothelial cells . The chemokine is involved in the recruitment of these immune cells to sites of inflammation or infection, thereby contributing to the body’s defense mechanisms .

Recombinant Mouse CXCL9/MIG protein is typically produced in E. coli . The recombinant protein is purified to high levels of purity, often exceeding 95% as determined by SDS-PAGE and HPLC . It is biologically active and can be used in various research applications, including chemotaxis assays .

The recombinant form of CXCL9/MIG is used extensively in immunological research to study its role in immune cell recruitment and activation . It is also utilized in assays to understand the mechanisms of inflammation and the immune response to infections and diseases .

Recombinant Mouse CXCL9/MIG protein is typically lyophilized and should be stored at -20°C to -70°C for long-term stability . Upon reconstitution, it is recommended to store the protein at 2-8°C for short-term use and at -20°C to -70°C for long-term storage . Repeated freeze-thaw cycles should be avoided to maintain protein integrity .