MIG Bovine
Escherichia Coli.
Small inducible cytokine B9, CXCL9, Gamma INF-induced monokine, MIG, chemokine (C-X-C motif) ligand 9, CMK, Humig, SCYB9, crg-10, monokine induced by gamma-INF.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
MIG (CXCL9) BovineRecombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 104 amino acids and having a molecular mass of approximately 18.0kDa.
MIG is purified by proprietary chromatographic techniques.
Product Specs
Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as Monokine induced by gamma INF (MIG), is a small cytokine in the CXC chemokine family. CXCL9 shares a close relationship with two other CXC chemokines, CXCL10 and CXCL11. The genes for these three chemokines are located near each other on human chromosome 4. CXCL9, CXCL10, and CXCL11 all exert their chemotactic effects by interacting with the CXCR3 chemokine receptor.
Recombinant Bovine MIG (CXCL9), produced in E. coli, is a non-glycosylated polypeptide chain composed of 104 amino acids. With a molecular weight of approximately 18.0 kDa, MIG undergoes purification using proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB (Phosphate Buffer) with 500 mM NaCl, at a pH of 7.0.
For reconstitution of lyophilized MIG (CXCL9), sterile 18 MΩ-cm H2O (water) is recommended. The minimum concentration should be no less than 100 µg/ml. Further dilutions can be made using other aqueous solutions.
While lyophilized MIG remains stable at room temperature for up to 3 weeks, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, MIG (CXCL9) should be stored at 4°C for a period of 2-7 days. For long-term storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity exceeds 96.0% as determined by:
(a) Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) analysis.
(b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
The biological activity, assessed through a chemotaxis bioassay employing human lymphocytes, ranges from 0.1 to 1.0 ng/ml.
Small inducible cytokine B9, CXCL9, Gamma INF-induced monokine, MIG, chemokine (C-X-C motif) ligand 9, CMK, Humig, SCYB9, crg-10, monokine induced by gamma-INF.
Escherichia Coli.
VPAIRNGRCS CINTSQGMIH PKSLKDLKQF APSPSCEKTE IIATMKNGNE ACLNPDLPEV KELIKEWEKQ VNQKKKQRKG KKYKKTKKVP KVKRSQRPSQ KKTT.
Product Science Overview
CXCL9 is primarily induced by interferon-gamma (IFN-γ) and acts as a T-cell chemoattractant . It plays a crucial role in the immune response by attracting T-cells to sites of inflammation or infection . This chemokine is closely related to two other CXC chemokines, CXCL10 and CXCL11, which also function as T-cell chemoattractants .
The bovine recombinant CXCL9 is a yeast-derived protein that is endotoxin-free and can be used in various applications such as cell culture, ELISA standards, and Western blot controls . The recombinant protein has a predicted molecular weight of 11.9 kDa and is produced in yeast to ensure it is naturally folded and post-translationally modified .
CXCL9 shows a high degree of homology across different species. For instance, it is 100% homologous in Bos taurus (cattle), Bison bison bison (bison), Bos indicus (zebu), and Bos mutus (wild yak) . This high level of conservation suggests that CXCL9 plays a vital role in the immune response across these species .
In summary, MIG (CXCL9) Bovine Recombinant is a crucial tool in immunological research, providing insights into the mechanisms of T-cell attraction and the broader immune response in cattle and related species.
