MAPRE3 Human

Microtubule-Associated Protein, RP/EB Family, Member 3 Human Recombinant
Cat. No.
BT12891
Source
Escherichia Coli.
Synonyms
RP3, EB3, EBF3, End-binding protein 3, EBF3-S, APC binding protein.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAPRE3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids (1-281a.a.) and having a molecular mass of 34.1kDa.
MAPRE3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MAPRE3, a microtubule-associated protein, plays a crucial role in microtubule regulation, cell polarity, and chromosome stability. It interacts with the tumor suppressor protein APC and influences microtubule dynamics. Primarily expressed in the central nervous system, MAPRE3 exhibits a strong association with APCL, a protein similar to the adenomatous polyposis coli tumor suppressor.
Description
This recombinant MAPRE3 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 301 amino acids (residues 1-281) with a molecular weight of 34.1 kDa. It includes an N-terminal 20 amino acid His-tag for purification purposes, which is achieved using proprietary chromatographic methods.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The MAPRE3 protein is supplied in a solution at a concentration of 1 mg/ml. The solution is buffered with 20mM Tris-HCl at pH 8.0, and contains 2mM DTT, 0.1M NaCl, and 10% glycerol.
Purity
Purity is determined to be greater than 90% as assessed by SDS-PAGE analysis.
Stability
For short-term storage (up to 2-4 weeks), the protein should be stored at 4°C. For longer-term storage, the protein should be aliquoted and stored frozen at -20°C. Repeated freeze-thaw cycles should be avoided.
Synonyms
RP3, EB3, EBF3, End-binding protein 3, EBF3-S, APC binding protein.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y

Product Science Overview

Introduction

Microtubule-Associated Protein, RP/EB Family, Member 3 (MAPRE3), also known as End-Binding Protein 3 (EB3), is a protein encoded by the MAPRE3 gene in humans. This protein is a member of the RP/EB family, which plays a crucial role in the regulation of microtubule dynamics. Microtubules are essential components of the cytoskeleton, involved in various cellular processes such as cell division, intracellular transport, and maintenance of cell shape.

Discovery and Function

MAPRE3 was first identified due to its association with the adenomatous polyposis coli (APC) protein, a tumor suppressor often mutated in colorectal cancer . The protein localizes to the cytoplasmic microtubule network and binds to the plus-end of microtubules, regulating their dynamics . It promotes microtubule growth and is involved in spindle function by stabilizing microtubules and anchoring them at centrosomes .

Role in Cellular Processes

During interphase, MAPRE3 is associated with the growing ends of microtubules, while during mitosis, it is found at the centrosomes and spindle microtubules . This localization suggests its involvement in the regulation of microtubule structures and chromosome stability. MAPRE3 also interacts with components of the dynactin complex and the intermediate chain of cytoplasmic dynein, indicating its role in intracellular transport .

Clinical Significance

Mutations or dysregulation of MAPRE3 have been linked to various diseases, including dyskeratosis congenita and signet ring cell adenocarcinoma . Its association with the APC protein also implicates it in colorectal cancer. Understanding the function and regulation of MAPRE3 is crucial for developing therapeutic strategies for these conditions.

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