MAP1LC3A Human

Microtubule-Associated Protein 1 Light Chain 3 Alpha Human Recombinant
Cat. No.
BT12437
Source
Escherichia Coli.
Synonyms
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, MLP3A.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAP1LC3A Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 128 amino acids (1-120 a.a.) and having a molecular mass of 15.1 kDa. The MAP1LC3A is fused to 8 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
MAP1LC3A, a light chain subunit that interacts with MAP1A and/or MAP1B, is involved in the formation of autophagosomal vacuoles. This protein plays a crucial role in neuronal development, maintaining a balance between plasticity and rigidity in neurons. Two alternatively spliced isoforms of MAP1LC3A are found in tissues like the testis, brain, heart, liver, and skeletal muscle, but not in the thymus or peripheral blood leukocytes.
Description
Recombinant Human MAP1LC3A, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 128 amino acids (including an 8-amino acid His-Tag at the C-terminus). It has a molecular weight of 15.1 kDa. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MAP1LC3A solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1M NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the product is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, MLP3A.
Source
Escherichia Coli.
Amino Acid Sequence
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG LEHHHHHH.

Product Science Overview

Gene and Protein Structure

The MAP1LC3A gene is located on chromosome 20 (20q11.22) in humans . It encodes a protein that is approximately 14 kDa in size and consists of 121 amino acids . The protein has two main isoforms resulting from alternative splicing . These isoforms can associate with either MAP1A or MAP1B, which are heavy chain subunits of the microtubule-associated proteins .

Function and Mechanism

MAP1LC3A is a ubiquitin-like modifier involved in the formation of autophagosomal vacuoles (autophagosomes) . Autophagy is a cellular degradation process where cells recycle cytoplasmic contents, including damaged organelles and proteins, to maintain cellular homeostasis . LC3A is essential for the elongation of the phagophore membrane, a precursor to the autophagosome .

The protein also interacts with the reticulophagy receptor TEX264, which is involved in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress . This interaction is crucial for the turnover of the endoplasmic reticulum through autophagy .

Clinical Significance

MAP1LC3A has been implicated in various diseases and conditions. For instance, its expression is suppressed in many tumor cell lines, suggesting a potential role in carcinogenesis . Additionally, diseases associated with MAP1LC3A include Cataract 13 with Adult I Phenotype and Diabetes Insipidus, Neurohypophyseal, X-Linked .

Research and Applications

Human recombinant MAP1LC3A is widely used in research to study autophagy and related cellular processes. It serves as a marker for autophagosomes and is used in various assays to monitor autophagic activity . The protein’s role in autophagy makes it a valuable target for studying neurodegenerative diseases, cancer, and other conditions where autophagy plays a critical role .

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