MAPRE1 Human
Microtubule-Associated Protein, RP/EB Family, Member 1 Human Recombinant
Cat. No.
BT12742
Source
Escherichia Coli.
Synonyms
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1, MGC117374, MGC129946.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MAPRE1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 288 amino acids (1-268 a.a.) and having a molecular mass of 32.2kDa. The MAPRE1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
MAPRE1 (EB1) is a member of the intermediate/early gene family. It was first discovered due to its interaction with the APC protein, a protein frequently mutated in both familial and sporadic colorectal cancer. MAPRE1 is found on microtubules, particularly at their growing ends, in interphase cells. During mitosis, it associates with centrosomes and spindle microtubules. This protein plays a role in microtubule polymerization and spindle function by stabilizing microtubules and anchoring them at centrosomes.
Description
Recombinant human MAPRE1, with a 20 amino acid His tag attached at its N-terminus, is produced in E. coli. This results in a single, non-glycosylated polypeptide chain of 288 amino acids (specifically, amino acids 1 to 268). The molecular weight of the recombinant protein is 32.2 kDa. Purification of MAPRE1 is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, sterile solution.
Formulation
The MAPRE1 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, 2mM DTT, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of MAPRE1 is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Microtubule-associated protein RP/EB family member 1, APC-binding protein EB1, End-binding protein 1, EB1, MAPRE1, MGC117374, MGC129946.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSDNLSRHDM LAWINESLQL NLTKIEQLCS GAAYCQFMDM LFPGSIALKK VKFQAKLEHE YIQNFKILQA GFKRMGVDKI IPVDKLVKGK FQDNFEFVQW FKKFFDANYD GKDYDPVAAR QGQETAVAPS LVAPALNKPK KPLTSSSAAP QRPISTQRTA AAPKAGPGVV RKNPGVGNGD DEAAELMQQV NVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG PQEEQEEY.
Product Science Overview
Discovery and Function
MAPRE1 was first identified through its interaction with the Adenomatous Polyposis Coli (APC) protein, which is often mutated in familial and sporadic forms of colorectal cancer . The protein localizes to microtubules, particularly at their growing ends, during the interphase of the cell cycle. During mitosis, MAPRE1 is associated with centrosomes and spindle microtubules .
Role in Cellular Processes
MAPRE1 is involved in several critical cellular processes:
- Microtubule Dynamics: It binds to the plus-end of microtubules, promoting their nucleation and elongation .
- Mitotic Spindle Positioning: It stabilizes microtubules and facilitates the dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation .
- Chromosome Stability: By associating with components of the dynactin complex and the intermediate chain of cytoplasmic dynein, MAPRE1 helps regulate microtubule structures and maintain chromosome stability .
Clinical Significance
Mutations or dysregulation of MAPRE1 have been linked to various diseases, including:
Research and Applications
Recombinant human MAPRE1 is widely used in research to study microtubule dynamics and their role in cell division and stability. Understanding the function and regulation of MAPRE1 can provide insights into the mechanisms underlying various diseases and potentially lead to the development of targeted therapies.
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