MAPK1 Human, His

Recombinant Human Mitogen-Activated Protein Kinase 1, His Tag
Cat. No.
BT11571
Source
Escherichia Coli.
Synonyms
Mitogen-activated protein kinase 1, EC 2.7.11.24, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, ERK, p38, p40, p41, ERK2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAPK1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 380 amino acids (1-360 a.a.) and having a molecular mass of 43.5 kDa. MAPK1 is fused to a 20 amino acids His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mitogen-activated protein kinase 1 (MAPK1), also called "extracellular signal-regulated kinase 2" (ERK2), is one of two closely related protein kinases (85% sequence identity). ERK1 and ERK2 were discovered during research into protein kinases rapidly phosphorylated following activation of cell surface tyrosine kinases such as the epidermal growth factor receptor. ERK phosphorylation activates their kinase activity. The molecular processes connecting cell surface receptors to ERK activation are multifaceted. Ras GTP-binding proteins were found to be involved in ERK activation. Another protein kinase, Raf-1, was shown to phosphorylate a "MAPK kinase," thus qualifying as a "MAPK kinase kinase." The "MAPK/ERK kinase" (MEK) was given to this MAPK kinase. Receptor-linked tyrosine kinases, Ras, Raf, MEK, and MAPK could be arranged in a signaling cascade that connects an extracellular signal to MAPK activation. MAPK1 knockout mice have significant developmental flaws.
Description
Recombinant human MAPK1, produced in E. coli, is a single, non-glycosylated polypeptide chain containing 380 amino acids (1-360 a.a.) with a molecular weight of 43.5 kDa. A 20 amino acid His-tag is fused to the N-terminus of MAPK1, which is then purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterile filtered.
Formulation
The MAPK1 solution is supplied in 20 mM Tris-HCl buffer (pH 8.0), 1mM DTT, and 10% glycerol.
Stability
If the entire vial will be used within 2-4 weeks, store at 4°C. For extended storage, freeze at -20°C. It is advised to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis determined purity to be greater than 95.0%.
Synonyms
Mitogen-activated protein kinase 1, EC 2.7.11.24, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, ERK, p38, p40, p41, ERK2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE MGSSHHHHHH SSGLVPRGSH MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase 1 (MAPK1), also known as Extracellular Signal-Regulated Kinase 2 (ERK2), is a crucial component of the MAP kinase signal transduction pathway. This pathway is involved in various cellular processes, including proliferation, differentiation, transcription regulation, and development . Recombinant Human MAPK1 with a His tag is a modified version of the protein that is produced using recombinant DNA technology and includes a polyhistidine tag (His tag) for purification purposes.

Structure and Function

MAPK1 is a serine/threonine kinase that plays a pivotal role in the MAPK/ERK cascade. This cascade is activated by various extracellular signals, leading to the phosphorylation and activation of MAPK1. Upon activation, MAPK1 translocates to the nucleus, where it phosphorylates various nuclear targets, influencing gene expression and cellular responses .

The His tag, typically consisting of six histidine residues, is added to the C-terminal or N-terminal end of the protein. This tag facilitates the purification of the recombinant protein using immobilized metal affinity chromatography (IMAC), which exploits the affinity of histidine residues for divalent metal ions like nickel or cobalt.

Production of Recombinant MAPK1

The production of recombinant MAPK1 involves several steps:

  1. Gene Cloning: The gene encoding MAPK1 is cloned into an expression vector, which includes a sequence for the His tag.
  2. Transformation: The expression vector is introduced into a suitable host cell, such as E. coli or insect cells.
  3. Expression: The host cells are cultured under conditions that induce the expression of the recombinant MAPK1 protein.
  4. Purification: The His-tagged MAPK1 is purified from the host cell lysate using IMAC. The His tag binds to the metal ions on the chromatography resin, allowing for the selective purification of the recombinant protein.
Applications

Recombinant Human MAPK1, His tag, is widely used in research to study the MAPK/ERK signaling pathway. It is utilized in various applications, including:

  • Biochemical Assays: To investigate the kinase activity and substrate specificity of MAPK1.
  • Drug Screening: To identify inhibitors or activators of MAPK1, which could be potential therapeutic agents.
  • Structural Studies: To determine the three-dimensional structure of MAPK1 and understand its interaction with other proteins and small molecules.

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