MAPK1 Antibody

Mitogen-Activated Protein Kinase 1, Mouse Anti Human
Cat. No.
BT20826
Source
Synonyms
Mitogen-activated protein kinase 1, EC 2.7.11.24, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, ERK, p38, p40, p41, ERK2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

Product Specs

Introduction
Mitogen-activated protein kinase 1 (MAPK1), also known as extracellular signal-regulated kinase 2 (ERK2), is one of two closely related protein kinases (85% sequence identity). Initially named ERK1 and ERK2, these kinases were discovered during research into proteins rapidly phosphorylated following the activation of cell surface tyrosine kinases like the epidermal growth factor receptor. ERK phosphorylation activates their kinase activity. The connection between cell surface receptors and ERK activation involves a complex molecular pathway. RasGTP-binding proteins have been implicated in ERK activation. Another protein kinase, Raf-1, phosphorylates a 'MAPK kinase,' thereby acting as a 'MAPK kinase kinase.' This MAPK kinase was designated as 'MAPK/ERK kinase' (MEK). Receptor-linked tyrosine kinases, Ras, Raf, MEK, and MAPK form a signaling cascade that transmits extracellular signals, leading to MAPK activation. Transgenic mice lacking MAPK1 exhibit significant defects in early development.
Formulation
1 mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
MAPK1 antibody has been validated for ELISA, Western blot, and Immunofluorescence analysis, ensuring its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. The recommended dilution range for Western blot and Immunofluorescence is 1:500 to 1:3000. A starting dilution of 1:500 is recommended.
Synonyms
Mitogen-activated protein kinase 1, EC 2.7.11.24, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, ERK, p38, p40, p41, ERK2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk.
Purification Method
MAPK1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1A4AT.
Immunogen
Anti-human MAPK1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human MAPK1 amino acids 1-360 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase 1 (MAPK1), also known as Extracellular Signal-Regulated Kinase 2 (ERK2), is a crucial component of the MAP kinase signal transduction pathway. This pathway is essential for various cellular processes, including proliferation, differentiation, transcription regulation, and development .

Structure and Function

MAPK1 is a serine/threonine kinase that requires phosphorylation by upstream kinases for activation. Upon activation, MAPK1 translocates to the nucleus, where it phosphorylates nuclear targets . This kinase is known for its dual functionality: it can act as a kinase and as a transcriptional repressor independent of its kinase activity .

Role in Cellular Processes

MAPK1 plays a significant role in integrating multiple biochemical signals. It is involved in various cellular processes such as:

  • Proliferation: MAPK1 is essential for cell division and growth.
  • Differentiation: It helps in the specialization of cells.
  • Transcription Regulation: MAPK1 influences the expression of various genes.
  • Development: It is crucial for the proper development of organisms .
Clinical Significance

MAPK1 has been implicated in several diseases, including cancer. Elevated expression of MAPK1 has been observed in human gastric cancer, where it promotes metastasis and invasion by regulating target genes . Additionally, MAPK1 is associated with Noonan Syndrome 13 and Specific Learning Disability .

Mouse Anti Human MAPK1 Antibody

The mouse anti-human MAPK1 antibody is commonly used in research to study the expression and function of MAPK1 in various human tissues. This antibody helps in detecting MAPK1 through various techniques such as Western blotting, immunohistochemistry, and flow cytometry.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

MAPK1 Human
Mitogen-Activated Protein Kinase 1 Human Recombinant
Cat. No.
BT11406
Source
Escherichia Coli.
MAPK1 Human, Active
Mitogen-Activated Protein Kinase 1 Human Recombinant, Active
Cat. No.
BT11487
Source
Escherichia Coli.
MAPK1 Human, His
Recombinant Human Mitogen-Activated Protein Kinase 1, His Tag
Cat. No.
BT11571
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.