LYVE1 Human

Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1 Human Recombinant
Cat. No.
BT7428
Source
Insect Cells.
Synonyms
Lymphatic vessel endothelial hyaluronic acid receptor 1 precursor, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Hyaluronic acid receptor, Extracellular link domain-containing protein 1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Soluble LYVE1 Human Recombinant fused to a C-terminal His-tag (6xHis) produced in baculovirus is a monomeric, glycosylated, polypeptide containing 232 amino acids (Met-1 to Gly 232) and having a total molecular mass of 25 kDa but as a result of glycosilation the Mw is 40 kDa.
The LYVE-1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
LYVE-1, a primary receptor for hyaluronan (HA), is located on the lymph vessel walls. This receptor plays a crucial role in HA binding. Structurally, LYVE-1 is a type I integral membrane polypeptide with 322 amino acids. It shares a 41% similarity with the CD44 HA receptor and possesses a single Link module, which is characteristic of the Link protein superfamily and is essential for HA binding. LYVE-1 binds to both soluble and immobilized HA. However, its unique characteristic lies in its specific localization to the lymph vessels, making it a distinctive marker for these vessels.
Description
Recombinant Human LYVE1, fused with a C-terminal His-tag (6xHis), is produced in a baculovirus expression system. It is a monomeric, glycosylated polypeptide with 232 amino acids (Met-1 to Gly 232). Although its molecular weight based on amino acid sequence is 25 kDa, glycosylation increases it to 40 kDa. The protein is purified using proprietary chromatographic techniques and supplied in a soluble form.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile filtered.
Formulation
LYVE1 was lyophilized from a sterile solution at a concentration of 1 mg/ml, without any additives.
Solubility
To reconstitute the lyophilized LYVE1, it is recommended to dissolve it in sterile water at a minimum concentration of 100 µg/ml. The reconstituted solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized sLYVE-1 remains stable for 3 weeks at room temperature. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. Once reconstituted, sLYVE-1 should be stored at 4°C for a period of 2-7 days. For future use, it should be stored below -18°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 95.0%, as determined by two methods: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Synonyms
Lymphatic vessel endothelial hyaluronic acid receptor 1 precursor, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Hyaluronic acid receptor, Extracellular link domain-containing protein 1.
Source
Insect Cells.

Product Science Overview

Structure and Function

LYVE1 is a Link domain-containing hyaladherin, which means it has a domain that allows it to bind to hyaluronic acid . It is homologous to CD44, the main HA receptor . LYVE1 acts as a receptor and binds to both soluble and immobilized hyaluronan . This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis .

Expression and Localization

LYVE1 is primarily expressed on the surface of lymphatic endothelial cells, making it a useful marker for these cells . However, its expression is not restricted to lymphatic vessels. It is also observed in normal liver blood sinusoids and embryonic blood vessels . Additionally, LYVE1 expression is found in a subset of macrophages, including those in the meninges of rats .

Physiological Role

The physiological role of LYVE1 is still under investigation, but its evolutionary conservation suggests it plays an important role . It is involved in the hyaluronan catabolic process, cell adhesion, and signal transduction . LYVE1 may act as a hyaluronan transporter, mediating its uptake for catabolism within lymphatic endothelial cells or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes .

Clinical Significance

LYVE1 has been implicated in various pathological conditions. It may have a role in tumor metastasis due to its involvement in lymphatic hyaluronan transport . Additionally, LYVE1+ macrophages have been observed in the synovium of patients with rheumatoid arthritis, suggesting a role in inflammatory processes .

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