LYVE1 Mouse Sf9
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
Description
The LYVE-1 is purified by proprietary chromatographic techniques.
Product Specs
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
Product Science Overview
The LYVE1 gene encodes a protein that consists of 228 amino acids and has a molecular mass of approximately 25 kDa. However, due to glycosylation, the molecular weight can increase to around 40 kDa . The protein is characterized by its ability to bind both soluble and immobilized hyaluronan, which is a significant component of the extracellular matrix .
LYVE1 plays a crucial role in the transport of hyaluronan within the lymphatic system. It may function either by mediating the uptake of hyaluronan for catabolism within lymphatic endothelial cells or by transporting it into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes . This receptor is also involved in the autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS) .
LYVE1 is predominantly expressed on the surface of lymphatic endothelial cells and serves as a marker for these cells. Its expression is not restricted to lymphatic vessels but is also observed in normal liver blood sinusoids and embryonic blood vessels . Additionally, LYVE1-positive macrophages have been identified in various tissues, including the meninges of rats .
The LYVE1 protein has been implicated in several physiological and pathological processes. It is known to play a role in lymphangiogenesis, which is the formation of new lymphatic vessels from pre-existing ones. This process is crucial for maintaining fluid balance and immune function. Moreover, LYVE1 has been associated with tumor metastasis, as it may facilitate the transport of cancer cells through the lymphatic system .
The recombinant form of LYVE1, produced in Sf9 insect cells, is often used in research to study its function and interactions. This recombinant protein is typically fused to a C-terminal His-tag (6xHis) to facilitate purification and detection. The recombinant LYVE1 retains its ability to bind hyaluronic acid and is used in various assays to investigate its role in lymphatic biology and disease .
