LYVE1 Human 25-235 a.a.
Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
LYVE1 Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 229 amino acids and having a molecular mass of 24.8 kDa. As a result of glycosylation, the LYVE1 migrates on SDS-PAGE at approximately 50 kDa.
LYVE1 is expressed with 15 amino acids His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Recombinant human LYVE1, spanning amino acids 25 to 235, has been expressed in insect cells and purified to yield a single, glycosylated polypeptide chain. This protein has a molecular weight of 24.8 kDa. Due to glycosylation, LYVE1 migrates at an apparent molecular weight of approximately 50 kDa on SDS-PAGE.
For purification purposes, LYVE1 has been engineered with a 15 amino acid His tag at its C-terminus and purified using proprietary chromatographic methods.
For short-term storage (up to 2-4 weeks), the LYVE1 protein solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal protein stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). Avoid repeated cycles of freezing and thawing.
The purity of LYVE1 is greater than 90%, as determined by two independent methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
DPLRAEELS IQVSCRIMGI TLVSKKANQQ LNFTEAKEAC RLLGLSLAGK DQVETALKAS FETCSYGWVG DGFVVISRIS PNPKCGKNGV GVLIRKVPVS RQFAAYCYNS SDTWTNSCIP EIITTKDPIF NTQTATQTTE FIVSDSTYSV ASPYSTIPAP TTTPPAPAST SIPRRKKLIC VTEVFMETST MSTETEPFVE NKAAFKNEAA GFGGSGRLVP RGSHHHHHH.
Product Science Overview
LYVE1 is characterized by its ability to bind to both soluble and immobilized hyaluronan (HA), a glycosaminoglycan involved in various biological processes . The receptor is composed of several domains, including the extracellular link domain, which is crucial for its binding activity . The specific fragment (25-235 a.a) refers to a portion of the protein that retains its functional properties when expressed recombinantly in human cells.
LYVE1 plays a significant role in the lymphatic system. It is primarily expressed on the surface of lymphatic endothelial cells and is involved in the transport of hyaluronan within the lymphatic vessels . This transport is essential for maintaining tissue fluid balance and facilitating immune cell trafficking .
Additionally, LYVE1 has been implicated in tumor metastasis. Its expression is often upregulated in various cancers, suggesting a role in the dissemination of tumor cells through the lymphatic system .
The receptor’s involvement in lymphangiogenesis (formation of new lymphatic vessels) makes it a valuable marker for studying lymphatic endothelial cells . It is also used in research to understand the mechanisms of diseases such as rheumatoid arthritis, where LYVE1+ macrophages have been observed in inflamed tissues .
The recombinant form of LYVE1, specifically the 25-235 amino acid fragment, is produced using human cell lines. This recombinant protein retains the functional properties of the native receptor, making it useful for various experimental applications, including studying receptor-ligand interactions and screening potential therapeutic agents.
