LRPAP1 Human
Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 Human Recombinant
Cat. No.
BT29608
Source
E.coli.
Synonyms
Low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1), A2MRAP, HBP44, RAP, A2RAP, Alpha-2-MRAP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
LRPAP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 348 amino acids (35-357) and having a molecular mass of 40.4kDa.
LRPAP1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LRPAP1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
LRPAP1, a member of the alpha-2-MRAP family, collaborates with LRP1/alpha-2-macroglobulin receptor and glycoprotein 330. This cell-surface protein forms a complex with the alpha 2 macroglobulin receptor's light and heavy chains, playing a role in the development of membrane glomerular nephritis.
Description
Recombinant human LRPAP1, produced in E. coli, is a non-glycosylated polypeptide chain containing 348 amino acids (residues 35-357) with a molecular weight of 40.4 kDa. This protein is expressed with a 25 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
LRPAP1 is supplied in a solution at a concentration of 1 mg/ml, containing 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Low density lipoprotein-related protein-associated protein 1 (alpha-2-macroglobulin receptor-associated protein 1), A2MRAP, HBP44, RAP, A2RAP, Alpha-2-MRAP.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMYSREK NQPKPSPKRE SGEEFRMEKL NQLWEKAQRL HLPPVRLAEL HADLKIQERD ELAWKKLKLD GLDEDGEKEA RLIRNLNVIL AKYGLDGKKD ARQVTSNSLS GTQEDGLDDP RLEKLWHKAK TSGKFSGEEL DKLWREFLHH KEKVHEYNVL LETLSRTEEI HENVISPSDL SDIKGSVLHS RHTELKEKLR SINQGLDRLR RVSHQGYSTE AEFEEPRVID LWDLAQSANL TDKELEAFRE ELKHFEAKIE KHNHYQKQLE IAHEKLRHAE SVGDGERVSR SREKHALLEG RTKELGYTVK KHLQDLSGRI SRARHNEL
Product Science Overview
Introduction
Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) is a multifunctional endocytic receptor that plays a crucial role in various physiological and pathological processes. It is a member of the LDL receptor family and is widely expressed in several tissues, including the brain, liver, and vascular smooth muscle cells .
Structure and Expression
LRP1 is a large receptor composed of a 515 kDa extracellular domain (ECD) and an 85 kDa intracellular domain (ICD). The ECD is responsible for ligand binding, while the ICD is involved in signal transduction . LRP1 is abundantly expressed in neurons, glial cells, smooth muscle cells, and pericytes in the cerebrovasculature .
Biological Functions
LRP1 is involved in a wide range of biological functions, including:
- Lipoprotein Homeostasis: LRP1 mediates the uptake of various lipoproteins, including apolipoprotein E (apoE) and α2-macroglobulin, playing a critical role in maintaining plasma lipoprotein levels .
- Atherosclerosis: LRP1 has a dual role in atherosclerosis. It helps remove atherogenic lipoproteins and proatherogenic ligands in the liver, but it also mediates the uptake of aggregated LDL, promoting foam cell formation and vascular wall transformation .
- Inflammation and Immune Response: LRP1 regulates inflammatory responses by modulating the release of cytokines and phagocytosis .
- Neuronal Regeneration and Survival: LRP1 is crucial for neuronal regeneration and survival, influencing synaptic integrity and function .
Pathological Implications
LRP1 is implicated in various diseases, including:
- Cardiovascular Diseases: LRP1’s role in lipoprotein homeostasis and atherosclerosis makes it a significant player in cardiovascular diseases. It is involved in vascular remodeling, foam cell biology, and inflammation .
- Alzheimer’s Disease: LRP1 is a major receptor for apoE and amyloid-β (Aβ), which are critical in Alzheimer’s disease pathogenesis. It regulates synaptic integrity and function, influencing the progression of neurodegenerative diseases .
- Acute Myocardial Infarction (AMI): LRP1 modulates the inflammatory response and tissue remodeling after AMI, providing a potential therapeutic target for reducing infarct size and cardiac dysfunction .
Therapeutic Potential
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