Leptin PAT7E10AT Antibody

Leptin Clone PAT7E10AT, Mouse Anti Human
Cat. No.
BT3282
Source
Synonyms

OB Protein, Obesity Protein, OBS, Obesity factor.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Leptin is a 16-kDa peptide hormone secreted by white adipocytes that plays a role in regulating food intake and energy balance. It acts as a crucial signal from fat cells in the feedback mechanism that controls body fat reserves.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The solution contains 1mg/ml of Leptin in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For extended periods, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The product is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The Leptin antibody (PAT7E10AT) has been validated for use in ELISA, Western blot analysis, and ICC/IF to ensure specificity and reactivity. However, it is recommended to optimize the antibody concentration for each specific application to achieve the best results.
Synonyms

OB Protein, Obesity Protein, OBS, Obesity factor.

Purification Method

Leptin antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT7E10AT.

Immunogen

Anti-human Leptin mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Leptin amino acids 22-167 purified from E. coli.

Ig Subclass

Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Leptin Clone PAT7E10AT

Leptin Clone PAT7E10AT is a monoclonal antibody produced in mice that specifically targets human leptin. This antibody is used in various research applications to study leptin’s role in metabolism, obesity, and related diseases.

Production and Characteristics
  • Clone: PAT7E10AT
  • Host: Mouse
  • Target: Human Leptin
  • Applications: This antibody has been validated for use in several laboratory techniques, including ELISA, Immunocytochemistry, Immunofluorescence, and Western Blot .
Applications in Research

The Mouse Anti Human Leptin Clone PAT7E10AT antibody is widely used in research to:

  1. Study Leptin’s Role in Metabolism: By targeting leptin, researchers can investigate how this hormone influences energy balance and fat storage.
  2. Obesity Research: Leptin is a key hormone in the regulation of body weight, and this antibody helps in studying its role in obesity and related metabolic disorders.
  3. Disease Mechanisms: Understanding leptin’s interaction with its receptors and other molecules can provide insights into various diseases, including diabetes and cardiovascular diseases.
Suppliers and Availability

This antibody is available from several suppliers, including Cedarlane and Biocompare . It is offered in different quantities to suit various research needs.

In summary, the Leptin Clone PAT7E10AT, Mouse Anti Human antibody is a valuable tool in the field of metabolic research, providing insights into the complex mechanisms of leptin and its role in health and disease.

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