Leptin Antibody
Description
Product Specs
Product Science Overview
Leptin is a 167 amino acid long protein hormone primarily involved in regulating body weight, metabolism, and reproductive function. It is encoded by the obese (ob) gene and is predominantly expressed by adipocytes (fat cells). Leptin plays a crucial role in signaling the brain about the body’s energy status, thereby influencing hunger and energy expenditure .
Leptin exerts its effects through leptin receptors, which are highly expressed in the hypothalamus, a brain region critical for regulating body weight. These receptors are also found in T lymphocytes and vascular endothelial cells. The interaction between leptin and its receptors helps regulate food intake, energy expenditure, and overall adiposity .
Mouse anti-human leptin antibodies are monoclonal antibodies developed in mice that specifically target human leptin. These antibodies are widely used in various scientific applications, including:
- Enzyme-Linked Immunosorbent Assay (ELISA): A technique used to detect and quantify soluble substances such as proteins, peptides, antibodies, and hormones.
- Western Blot: A method used to detect specific proteins in a sample.
- Immunohistochemistry (IHC): A technique for identifying cellular or tissue components by using labeled antibodies as specific reagents.
- Immunocytochemistry (ICC): Similar to IHC but used for cells.
- Flow Cytometry: A technology used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser .
The use of mouse anti-human leptin antibodies is crucial in research focused on understanding leptin’s role in various physiological and pathological processes. These antibodies help in:
- Studying Metabolic Disorders: Leptin is a key player in obesity and metabolic syndrome research.
- Investigating Reproductive Health: Leptin’s role in reproductive function makes it a target for studies related to fertility and pregnancy.
- Exploring Immune Function: Leptin’s interaction with T lymphocytes is significant in immunological research .
