LAG-1 Human
LAG-1 Human Recombinant (CCL4L1)
Cat. No.
BT19807
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 4-like, Lymphocyte activation gene 1 protein, LAG-1, Macrophage inflammatory protein 1-beta, MIP-1-beta, Monocyte adherence-induced protein 5-alpha, Small-inducible cytokine A4-like, CCL4L1, CCL4L, LAG1, SCYA4L1, CCL4L2, SCYA4L2, AT744.2.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
LAG-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 69 amino acids and having a molecular mass of 7.8kDa.
The CCL4L1 is purified by proprietary chromatographic techniques.
The CCL4L1 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
CCL4L1 (C-C motif chemokine 4-like) belongs to the intercrine beta (chemokine CC) family. This protein shares similarities with CCL4, known to inhibit HIV replication in CCR5-expressing peripheral blood monocytes.
Description
LAG-1 Human Recombinant, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 69 amino acids. With a molecular weight of 7.8 kDa, it undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
LAG-1 was lyophilized from a 0.2µm filtered solution concentrated in PBS with a pH of 7.4.
Solubility
For reconstitution of lyophilized LAG-1, it is recommended to use sterile 18M-cm H₂O at a concentration not less than 100µg/ml. Further dilutions can be made using other aqueous solutions.
Stability
Lyophilized LAG-1 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, CCL4L1 should be stored at 4°C for 2-7 days. For long-term storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The ED₅₀, determined through a cell proliferation assay using human CCR5 transfected murine BaF3 cells, is less than 2.0 ng/ml. This corresponds to a specific activity greater than 5.0 x 10⁵ IU/mg.
Synonyms
C-C motif chemokine 4-like, Lymphocyte activation gene 1 protein, LAG-1, Macrophage inflammatory protein 1-beta, MIP-1-beta, Monocyte adherence-induced protein 5-alpha, Small-inducible cytokine A4-like, CCL4L1, CCL4L, LAG1, SCYA4L1, CCL4L2, SCYA4L2, AT744.2.
Source
Escherichia Coli.
Amino Acid Sequence
APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN.
Product Science Overview
Structure and Composition
LAG-1 is a small protein with a molecular weight of approximately 7.7 kDa . It consists of 69 amino acid residues . The protein is nearly identical to MIP-1β (ACT II isotype), differing only by two amino acid substitutions: arginine for histidine at position 22 and serine for glycine at position 47 .
Biological Functions
Production and Purification
Applications in Research
Stability and Storage
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