KRT18 Human, His
Description
The KRT18 is fused to a His Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Cytokeratin 18 (CK18) is a type I intermediate filament protein that is primarily expressed in epithelial tissues. It plays a crucial role in maintaining the structural integrity of epithelial cells and is involved in various cellular processes such as apoptosis, cell signaling, and differentiation. The recombinant form of Cytokeratin 18, tagged with a His (histidine) tag, is widely used in research for various applications, including protein-protein interaction studies, immunoassays, and as a control in biochemical experiments.
Cytokeratin 18 is composed of 430 amino acids and has a molecular weight of approximately 48 kDa . The recombinant form of CK18 is expressed in mammalian cells to ensure proper folding and post-translational modifications, which are essential for its biological activity . The His tag, typically consisting of six histidine residues, is added to the N-terminus of the protein to facilitate purification and detection .
CK18, along with its partner Cytokeratin 8 (CK8), forms a heterodimer that contributes to the cytoskeletal network in epithelial cells. This network provides mechanical support and helps maintain cell shape and integrity. CK18 is also involved in several cellular processes, including:
- Apoptosis: During apoptosis, CK18 is cleaved by caspases, which serves as a marker for cell death.
- Cell Signaling: CK18 interacts with various signaling molecules, influencing pathways related to cell growth, differentiation, and stress responses.
- Barrier Protection: CK18, in conjunction with CK8, plays a role in interleukin-6 (IL-6)-mediated barrier protection .
The recombinant form of CK18 with a His tag is utilized in various research applications:
- Protein-Protein Interaction Studies: The His tag allows for easy purification and immobilization of CK18, facilitating the study of its interactions with other proteins.
- Immunoassays: CK18 can be used as a standard or control in ELISA and Western blot assays to detect and quantify CK18 levels in biological samples.
- Biochemical Experiments: The recombinant protein serves as a positive control in experiments involving protein folding, stability, and post-translational modifications .
