KLF4 Human

Kruppel-Like Factor 4 Human Recombinant
Cat. No.
BT1368
Source
E.coli.
Synonyms
Kruppel-like factor 4 (gut), EZF, GKLF, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, endothelial Kruppel-like zinc finger protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KLF4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 545 amino acids (11-395) and having a molecular mass of 58.1 kDa.
The KLF4 is fused to a 159 amino acid His-CaM Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
KLF4 is a transcription factor that can act as both an activator and a repressor of gene expression. It is primarily found in erythroid tissues and the gut. KLF4 plays a role in epithelial cell differentiation, as well as skeletal and kidney development.
Description
Recombinant human KLF4 protein was expressed in E. coli and purified. It is a single, non-glycosylated polypeptide chain containing amino acids 11-395 of KLF4 and a 159 amino acid His-CaM tag at the N-terminus. The molecular weight of the protein is 58.1 kDa. Purification was achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The KLF4 protein is supplied at a concentration of 1 mg/ml in a solution containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the KLF4 protein is greater than 80% as determined by SDS-PAGE analysis.
Synonyms
Kruppel-like factor 4 (gut), EZF, GKLF, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, endothelial Kruppel-like zinc finger protein.
Source
E.coli.
Amino Acid Sequence
MAHHHHHHMA DQLTEEQIAE FKEAFSLFDK DGDGTITTKE LGTVMRSLGQ NPTEAELQDM INEVDADGNG TIDFPEFLTM MARKMKDTDS EEEIREAFRV FDKDGNGYIS AAELRHVMTN LGEKLTDEEV DEMIREADID GDGQVNYEEF VQMMTAKGSM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV PPLHYQELMP PGSCMPEEPK PKRGRRSWPR KRTAT

Product Science Overview

Discovery and Evolution

KLF4 was first isolated by Shields et al. in 1996 from a NIH3T3 cDNA library . The gene is conserved among vertebrate species, from zebrafish to humans . Since its discovery, KLF4 has gained significant attention, particularly after it was identified in 2006 as one of the four key factors required for the induction of pluripotent stem cells (iPSCs) .

Structure and Function

KLF4 contains three C2H2-type zinc fingers at the carboxyl terminus, which preferentially bind to GC-rich cis-DNA elements . This structure allows KLF4 to function as both an activator and repressor of gene expression . KLF4 is predominantly expressed in terminally differentiated epithelial tissues, including the skin, lung, and gastrointestinal tract .

Roles in Cellular Processes

KLF4 is involved in various cellular processes:

  • Proliferation: KLF4 regulates cellular proliferation by binding to specific DNA sequences, such as CACCC boxes and GC boxes .
  • Differentiation: It plays a role in the differentiation of epithelial cells and is involved in skeletal and kidney development .
  • Apoptosis: KLF4 is also implicated in the regulation of apoptosis, contributing to the maintenance of cellular homeostasis .
  • Somatic Cell Reprogramming: KLF4 is one of the four factors (along with Oct4, Sox2, and c-Myc) used to reprogram somatic cells into iPSCs .
Clinical Significance

KLF4 has been studied extensively for its role in various diseases, including cancer. It has been shown to have different mechanisms in different tumors, but its prognostic role remains unclear . Despite the rapid progression in cancer research, the expression of KLF4 has not been consistently associated with prognosis for overall survival, disease-free survival, or disease-specific survival .

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