KLF4 Antibody
Krueppel-like factor 4, Mouse Anti Human
Cat. No.
BT22054
Source
Synonyms
Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
KLF4, also known as gut-enriched Kruppel-like factor (GKLF), is a zinc finger transcription factor belonging to the Kruppel-like family. This factor plays a crucial role in epithelial cell proliferation and differentiation, particularly in the gut, and is associated with growth arrest. KLF4 is also involved in cell cycle regulation by inhibiting DNA synthesis and blocking the G1/S phase transition.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
Solution containing 1mg/ml of KLF4 antibody in PBS, pH 7.4, with 0.1% sodium azide.
Storage Procedures
For storage up to 1 month, keep at 4°C. For longer-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C or 1 month at 4°C.
Applications
The KLF4 antibody has undergone ELISA and Western blot analysis to confirm its specificity and reactivity. However, optimal working dilutions may vary depending on the specific application. For Western blot analysis, a dilution range of 1:1000 to 1:2000 is recommended, with a starting dilution of 1:1000.
Synonyms
Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4, EZF, GKLF.
Purification Method
KLF4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4E6AT.
Immunogen
Anti-human KLF4 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human KLF4 amino acids 1-170 purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and κ light chain.
Product Science Overview
Introduction to Krüppel-like Factor 4 (KLF4)
Krüppel-like factor 4 (KLF4) is a zinc finger transcription factor that plays a crucial role in regulating various cellular processes. It was originally isolated from an NIH3T3 cDNA library in 1996 . KLF4 is highly conserved across species, from zebrafish to humans, and is expressed in numerous tissues, including intestinal epithelial cells, skin, and neural stem cells .
Functions and Mechanisms
KLF4 is involved in controlling multiple biological pathways, such as:
- Proliferation: KLF4 regulates cell cycle progression and can inhibit cell proliferation following DNA damage .
- Differentiation: It plays a significant role in the differentiation of various cell types, including neural stem cells .
- Neuroinflammation: KLF4 is implicated in the activation of microglia, the resident macrophages of the central nervous system (CNS), which is a hallmark of neuroinflammation in neurodegenerative diseases .
- Oxidative Stress and Apoptosis: KLF4 is involved in the cellular response to oxidative stress and can regulate apoptosis .
Role in Diseases
KLF4 has been studied in the context of various diseases:
- Neurodegenerative Diseases: KLF4 is involved in the regulation of neuroinflammation and has been linked to diseases such as Parkinson’s disease .
- Autoimmune Diseases: Increased expression of KLF4 has been observed in synovial tissue from rheumatoid arthritis (RA) patients .
- Cancer: KLF4 dysregulation has been associated with genetic instability and cancer progression .
Mouse Anti-Human KLF4 Antibody
Mouse anti-human KLF4 antibodies are used in research to study the expression and function of KLF4 in human tissues. These antibodies are typically generated by immunizing mice with human KLF4 protein, leading to the production of antibodies that specifically recognize and bind to human KLF4.
Applications in Research
Mouse anti-human KLF4 antibodies are valuable tools in various research applications, including:
- Western Blotting: To detect KLF4 protein levels in different tissues and cell lines.
- Immunohistochemistry: To visualize KLF4 expression in tissue sections.
- Flow Cytometry: To analyze KLF4 expression in different cell populations.
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