KLF4 Human, His
Description
Product Specs
Product Science Overview
Kruppel-Like Factor 4 (KLF4) is a transcription factor that belongs to the Kruppel family of zinc finger proteins. It plays a crucial role in regulating various cellular processes, including differentiation, proliferation, and apoptosis. KLF4 is particularly important in maintaining the barrier function of the skin and in the regulation of the cell cycle, especially in response to DNA damage by mediating the tumor suppressor gene p53 .
The human recombinant KLF4 protein with a His tag is a denatured recombinant protein that includes a N-terminal His-tag and corresponds to the amino acids 11-395 of the human KLF4 protein . This recombinant protein is produced in Escherichia coli (E. coli) and has a molecular weight of approximately 44.2 kDa . The His tag facilitates the purification of the protein using affinity chromatography techniques.
The recombinant KLF4 protein is typically prepared using bacterial expression systems, such as E. coli. The gene encoding KLF4 is cloned into an expression vector that includes a sequence for the His tag. This vector is then introduced into E. coli cells, which express the recombinant protein. The protein is subsequently purified using affinity chromatography, which exploits the interaction between the His tag and a nickel or cobalt resin .
KLF4 functions as both an activator and a repressor of transcription. It binds to the 5’-CACCC-3’ core sequence in the promoter regions of target genes, regulating their expression. KLF4 is involved in various biological processes, including the maintenance of embryonic stem cells, prevention of their differentiation, and the establishment of the barrier function of the skin . It also plays a role in the differentiation of epithelial cells and may be involved in skeletal and kidney development .
The recombinant KLF4 protein with a His tag is widely used in research to study its function and regulation. It is particularly useful in experiments involving protein-protein interactions, DNA-binding studies, and the investigation of its role in various cellular processes. The His tag allows for easy purification and detection of the protein, making it a valuable tool in molecular biology and biochemistry research .
