KLF12 Human

Kruppel-Like Factor 12 (KLF12) is a member of the Kruppel-like zinc finger protein family, which plays a crucial role in regulating gene expression. This family of transcription factors is characterized by the presence of C2H2-type zinc finger motifs that bind to GC-rich regions in DNA. KLF12, in particular, is known for its ability to repress the expression of the AP-2 alpha gene by binding to a specific site in its promoter region .

KLF12 Human Recombinant is produced in Escherichia coli as a single, non-glycosylated polypeptide chain containing 425 amino acids. The recombinant protein has a molecular mass of 46.6 kDa and is fused to a 23 amino acid His-tag at the N-terminus. The protein is purified using proprietary chromatographic techniques to ensure high purity and stability .

KLF12 functions as a transcriptional repressor by interacting with corepressors such as CtBP1. It binds to the promoter regions of target genes, thereby inhibiting their transcription. This repression mechanism is vital for regulating various cellular processes, including proliferation, differentiation, and apoptosis .

KLF12 is involved in the regulation of gene expression during vertebrate development and carcinogenesis. Its ability to repress the AP-2 alpha gene, a developmentally regulated transcription factor, highlights its importance in developmental biology. Additionally, KLF12 has been implicated in various diseases, including cancer, where its dysregulation can lead to abnormal cell growth and tumor formation .

The recombinant form of KLF12 is widely used in laboratory research to study its function and regulatory mechanisms. It is particularly useful in experiments aimed at understanding gene expression, transcriptional regulation, and the role of KLF12 in disease pathogenesis. The availability of high-purity recombinant KLF12 allows researchers to conduct detailed biochemical and structural analyses, furthering our understanding of this important transcription factor .