KGF Human, Plant
Rice Grain
Greater than 95% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
KGF Human Recombinant produced in Oryza Sativa is a single, polypeptide chain having a molecular mass of approximately 19.2kDa. The KGF is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human KGF, produced in rice grain (Oryza Sativa), is a single polypeptide chain with an approximate molecular weight of 19.2kDa. The purification of KGF is achieved through specialized chromatographic methods.
KGF is lyophilized from a 0.2µm filtered solution without any added stabilizers.
To reconstitute the lyophilized KGF, it is recommended to use sterile 18MΩ-cm water at a concentration of 100µg/ml. This solution can then be diluted further using other aqueous solutions as needed.
Lyophilized KGF remains stable at room temperature for up to 3 weeks; however, for long-term storage, it is recommended to store it desiccated below -18°C. Once reconstituted, FGF7 should be stored at 4°C for 2-7 days. For extended storage, freezing below -18°C is advised. To enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity of KGF is determined to be greater than 95% using SDS-PAGE analysis.
The biological activity of KGF is comparable to standard KGF. Its activity is measured by its ability to stimulate the proliferation of HUVECs in a dose-dependent manner, with a typical effective concentration of 10ng/ml. This corresponds to a specific activity of 100,000 units/mg.
Rice Grain
Product Science Overview
Recombinant human KGF (rhKGF) is a synthetic version of the naturally occurring KGF. It is produced using recombinant DNA technology, which involves inserting the gene responsible for KGF production into a host organism, such as bacteria, yeast, or plant cells . This allows for large-scale production of the protein for therapeutic use.
The production of rhKGF in plant systems is a relatively recent advancement. One notable method involves using transgenic plant cell cultures, such as those derived from hazelnut (Corylus avellana L.) . In this system, the rhKGF gene is introduced into the plant cells, which then produce the protein. This method offers several advantages, including a high growth rate of the plant cells and the ability to produce significant amounts of the protein within a few weeks .
Therapeutic Uses: rhKGF is used in various medical treatments, particularly for conditions involving epithelial cell damage. For example, it has been successfully used to treat severe, refractory hemorrhagic cystitis following allogeneic hematopoietic cell transplantation . It is also used to promote healing of mucositis and wounds, which are common side effects of chemotherapy .
Advantages of Plant-Based Production: Producing rhKGF in plant systems offers several benefits over traditional methods. It is cost-effective, scalable, and reduces the risk of contamination with human pathogens. Additionally, plant-based systems can be engineered to optimize protein production and secretion, making them a viable option for large-scale manufacturing .
