KCTD5 Human

Potassium Channel Tetramerisation Domain Containing 5 Human Recombinant
Cat. No.
BT5292
Source
E.coli.
Synonyms
BTB/POZ domain-containing protein KCTD5, KCTD5.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KCTD5 Human Recombinant produced in E. coli is a single polypeptide chain containing 257 amino acids (1-234) and having a molecular mass of 28.5 kDa. KCTD5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
KCTD5 (BTB/POZ domain-containing protein KCTD5) is a 234-amino acid protein primarily found in the cytoplasm. Upon interaction with REP proteins, it relocates to the nucleus. T-cell receptor stimulation of peripheral blood lymphocytes leads to increased KCTD5 expression post-transcriptionally. Notably, KCTD5 interacts with cullin3, facilitates the attachment of ubiquitinated proteins, and forms oligomers through its BTB domain.
Description
Recombinant human KCTD5, produced in E. coli, is a single polypeptide chain comprising 257 amino acids (residues 1-234) with a molecular weight of 28.5 kDa. The protein includes a 23-amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The KCTD5 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 40% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
BTB/POZ domain-containing protein KCTD5, KCTD5.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAENHCE LLSPARGGIG AGLGGGLCRR CSAGLGALAQ RPGSVSKWVR LNVGGTYFLT TRQTLCRDPK SFLYRLCQAD PDLDSDKDET GAYLIDRDPT YFGPVLNYLR HGKLVINKDL AEEGVLEEAE FYNITSLIKL VKDKIRERDS KTSQVPVKHV YRVLQCQEEE LTQMVSTMSD GWKFEQLVSI GSSYNYGNED QAEFLCVVSK ELHNTPYGTA SEPSEKAKIL QERGSRM

Product Science Overview

Gene and Protein Structure

The KCTD5 gene is located on chromosome 16 and encodes a protein that is involved in various cellular processes. The protein contains a BTB/POZ domain, which is known for its role in protein-protein interactions. This domain is crucial for the tetramerisation of potassium channels, allowing them to form functional tetrameric structures .

Function and Mechanism

KCTD5 is predicted to be involved in the proteasome-mediated ubiquitin-dependent protein catabolic process. This means it helps in the degradation of proteins that are tagged with ubiquitin, a small regulatory protein. The interaction of KCTD5 with CUL3 suggests that it may act as a substrate adapter in some E3 ligase complexes .

Interestingly, KCTD5 does not affect the function of several potassium channels, including Kv2.1/KCNB1, Kv1.2/KCNA2, Kv4.2/KCND2, and Kv3.4/KCNC4 . This indicates that its role might be more specialized or context-dependent.

Clinical Significance

Mutations or dysregulation of the KCTD5 gene have been associated with certain diseases. For example, it is linked to Brachydactyly, Type D, a condition characterized by shortening of the fingers and toes. It is also associated with Immunodeficiency, Common Variable, 10, which affects the immune system .

Research and Applications

The study of KCTD5 is ongoing, with researchers exploring its various roles in cellular processes and its potential implications in disease. The recombinant form of this protein is used in research to better understand its function and to develop potential therapeutic interventions.

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