KCTD11 Human

Potassium Channel Tetramerisation Domain Containing 11 Human Recombinant
Cat. No.
BT5067
Source
E.coli.
Synonyms
C17orf36, KCASH1, MGC129844, REN, REN/KCTD11, KCTD11, BTB/POZ domain-containing protein KCTD11.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KCTD11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232a.a) and having a molecular mass of 28kDa. KCTD11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Potassium Channel Tetramerisation Domain Containing 11 (KCTD11) is a 232-amino acid protein involved in regulating neuronal differentiation. It promotes growth arrest and apoptosis (programmed cell death) and increases the expression of p27, a protein that inhibits cell cycle progression. KCTD11 is found in highest concentrations in the cerebellum (a brain region responsible for motor control and coordination). It acts by inhibiting the Hedgehog signaling pathway, which is crucial for embryonic development and cell growth, and activating the caspase cascade, a pathway leading to apoptosis. KCTD11 is associated with signaling pathways such as Melatonin Signaling and Activation of cAMP-Dependent PKA.
Description
This product consists of the human KCTD11 protein, recombinantly produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain comprising 252 amino acids (specifically, amino acids 1 through 232 of the native protein sequence). A 20-amino acid His-tag is attached to the N-terminus to facilitate purification. The molecular weight of the recombinant protein is 28 kDa. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The KCTD11 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea (a chaotropic agent to increase solubility), and 10% glycerol (a cryoprotectant).
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as albumin (HSA or BSA) to a final concentration of 0.1% is recommended for long-term storage. Avoid repeated freezing and thawing of the product.
Purity
The purity of the KCTD11 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
C17orf36, KCASH1, MGC129844, REN, REN/KCTD11, KCTD11, BTB/POZ domain-containing protein KCTD11.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLGAMFRAGT PMPPNLNSQG GGHYFIDRDG KAFRHILNFL RLGRLDLPRG YGETALLRAE ADFYQIRPLL DALRELEASQ GTPAPTAALL HADVDVSPRL VHFSARRGPH HYELSSVQVD TFRANLFCTD SECLGALRAR FGVASGDRAE GSPHFHLEWA PRPVELPEVE YGRLGLQPLW TGGPGERREV VGTPSFLEEV LRVALEHGFR LDSVFPDPED LLNSRSLRFV RH.

Product Science Overview

Structure and Function

KCTD11 is a protein-coding gene that produces a protein involved in several important cellular processes. The protein contains a BTB/POZ domain, which is known for its role in protein-protein interactions . This domain is crucial for the tetramerisation of potassium channels, which are essential for maintaining the electrical excitability of cells .

The KCTD11 protein is involved in the regulation of neuronal differentiation and has been shown to induce apoptosis (programmed cell death), growth arrest, and the expression of cyclin-dependent kinase inhibitor CDKN1B . It acts as a tumor suppressor and inhibits the growth and tumorigenicity of medulloblastoma, a type of brain cancer .

Pathways and Interactions

KCTD11 is upregulated by various neurogenic signals, such as retinoic acid, epidermal growth factor (EGF), and nerve growth factor (NGF) . It functions as an antagonist of the Hedgehog signaling pathway, which is involved in cell proliferation and differentiation . By affecting the nuclear transfer of the transcription factor GLI1, KCTD11 helps maintain cerebellar granule cells in an undifferentiated state .

Additionally, KCTD11 is a probable substrate-specific adapter for a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex towards HDAC1 . This interaction is significant for the regulation of protein degradation and cellular homeostasis.

Clinical Significance

Mutations or dysregulation of the KCTD11 gene have been associated with several diseases, including medulloblastoma and brachydactyly type D . The protein’s role as a tumor suppressor makes it a potential target for cancer therapy, particularly in the treatment of medulloblastoma .

Recombinant KCTD11

Recombinant KCTD11 is produced using Escherichia coli (E. coli) expression systems. The recombinant protein is typically a non-glycosylated polypeptide chain containing 252 amino acids and has a molecular mass of approximately 28 kDa . It is often fused to a His-tag at the N-terminus to facilitate purification using chromatographic techniques .

Recombinant KCTD11 is used in various research applications, including studies on protein-protein interactions, signal transduction pathways, and the development of potential therapeutic agents targeting the Hedgehog signaling pathway .

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