IL22 Human, Sf9

Interleukin-22 Mouse Recombinant, Sf9
Cat. No.
BT12687
Source

Sf9, Baculovirus cells.

Synonyms
IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.
Appearance

Sterile filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL22 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (34-179 a.a.)  fused to a 9 aa His Tag at C-terminus containing a total of 155 amino acids and having a molecular mass of 17.8kDa.

IL22 shows multiple bands between 18-28kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF), is a cytokine that plays a role in inflammatory responses. It is produced by activated T cells and other immune cells, and acts on cells in various tissues, including the liver, intestines, and skin. IL-22 signals through a receptor complex composed of IL-22R and IL-10Rβ, leading to the activation of STAT signaling pathways and the expression of genes involved in inflammation, tissue repair, and antimicrobial defense.
Description
IL-22, produced in Sf9 insect cells, is a glycosylated protein with a molecular weight of approximately 17.8 kDa. The recombinant protein consists of amino acids 34-179 of the mature IL-22 sequence, with a 9-amino acid His tag at the C-terminus. SDS-PAGE analysis reveals multiple bands between 18-28 kDa under reducing conditions, likely representing different glycosylation patterns. The protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, colorless solution.
Formulation
The IL-22 protein solution is provided at a concentration of 1 mg/ml in a buffer containing phosphate-buffered saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the IL-22 protein solution can be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. To prevent protein degradation during freezing and thawing, it is advisable to add a carrier protein such as albumin (0.1% HSA or BSA). Repeated freezing and thawing should be avoided.
Purity
The purity of the IL-22 protein is greater than 90%, as determined by SDS-PAGE analysis.
Biological Activity
The biological activity of the IL-22 protein was assessed by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50, defined as the concentration of IL-22 required to achieve half-maximal IL-10 secretion, was determined to be approximately 1.2 ng/ml.
Synonyms
IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23.
Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPAPISSHC RLDKSNFQQP YITNRTFMLA KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACIH HHHHH.

Product Science Overview

Biological Functions of IL-22

IL-22 is primarily produced by immune cells such as Th17, Th22, and innate lymphoid cells . It acts on non-hematopoietic cells, including epithelial cells and fibroblasts, to mediate its effects. The primary functions of IL-22 include:

  • Epithelial Cell Protection: IL-22 helps protect epithelial cells from damage and promotes their regeneration .
  • Inflammation Regulation: It plays a role in modulating inflammatory responses, particularly in chronic inflammatory diseases .
  • Host Defense: IL-22 is involved in the defense against bacterial infections by enhancing the barrier function of epithelial cells .
Recombinant IL-22 Production

Recombinant IL-22 (Mouse) is produced using the Sf9 insect cell expression system. This system is widely used for the production of recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells. The process involves the following steps:

  1. Gene Cloning: The gene encoding mouse IL-22 is cloned into a suitable expression vector.
  2. Transfection: The vector is introduced into Sf9 insect cells, which are then cultured to express the recombinant protein.
  3. Protein Purification: The expressed IL-22 protein is purified using various chromatographic techniques to obtain a high-purity product.
Applications of Recombinant IL-22

Recombinant IL-22 has several applications in research and therapeutic development:

  • Inflammatory Disease Models: It is used in animal models to study the role of IL-22 in various inflammatory diseases, such as atherosclerosis and colitis .
  • Tissue Repair Studies: Researchers use recombinant IL-22 to investigate its potential in promoting tissue repair and regeneration .
  • Drug Development: IL-22 is being explored as a potential therapeutic agent for conditions involving epithelial damage and chronic inflammation .

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