IL 22 Rat

IL-22 was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. It is also known as IL-10-related T cell-derived inducible factor (IL-TIF) . IL-22 is produced by T lymphocytes and plays a crucial role in the immune response by inhibiting IL-4 production by Th2 cells and inducing acute phase reactants in the liver and pancreas .

IL-22 signals through a receptor system consisting of IL-10R-beta/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family . The biological activity of IL-22 is measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells), with an expected ED50 for this effect being 0.15-0.75 ng/ml .

Recombinant Rat IL-22 is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of approximately 16.6 kDa . The recombinant protein is typically lyophilized from a 0.2 µm filtered solution in PBS and can be reconstituted in sterile water or PBS for use in various applications .

Recombinant IL-22 is used in laboratory research to study its role in immune responses and its potential therapeutic applications. It is particularly noted for its role in promoting antimicrobial defense and preventing epithelial damage . The lyophilized form of IL-22 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for future use, avoiding repeated freeze-thaw cycles .