IL 22 Mouse, PEG

Interleukin-22 (IL-22) is a cytokine that plays a crucial role in the immune response, particularly in the regulation of inflammatory processes and the maintenance of tissue homeostasis. It is also known as IL-10-related T cell-derived inducible factor (IL-TIF). IL-22 was initially identified as a gene induced by IL-9 in mouse T cells and mast cells .

Mouse IL-22 cDNA encodes a protein consisting of 179 amino acid residues, including a 33 amino acid signal peptide that is cleaved to generate a mature protein of 147 amino acids . The recombinant form of IL-22 is produced in Escherichia coli (E. coli) and is a single, non-glycosylated homodimeric polypeptide chain .

Pegylation refers to the process of attaching polyethylene glycol (PEG) chains to a protein or peptide. This modification enhances the stability and solubility of the protein, reduces immunogenicity, and prolongs its half-life in the bloodstream. The pegylated form of mouse IL-22 is mono-pegylated with a 20 kDa PEG chain . This modification results in a protein with a molecular mass of 36 kDa, which appears as a 50 kDa protein on SDS-PAGE due to its enlarged hydrodynamic volume .

IL-22 is known to activate the STAT-1 and STAT-3 signaling pathways in various cell types, including hepatoma cell lines. It upregulates the production of acute-phase proteins and is involved in inflammatory responses . The functional IL-22 receptor complex consists of two subunits: IL-22R and IL-10Rβ, both of which belong to the class II cytokine receptor family .

Recombinant pegylated mouse IL-22 is used in various research applications, including studies on inflammation, immune response, and tissue regeneration. Its enhanced stability and prolonged half-life make it suitable for long-term infusion studies using osmotic pumps .

Lyophilized pegylated mouse IL-22 is stable at room temperature for several days but should be stored desiccated below -20°C for long-term storage. Upon reconstitution, the protein can be stored at 4°C for several weeks, making it suitable for extended experimental use .