Influenza-A H5N1 Antibody
Influenza-A Hemagglutinin H5N1, Mouse Antibody
Cat. No.
BT8342
Source
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified avian influenza virus type A H5N1.
Product Specs
Introduction
Influenza A virus subtype H5N1, commonly known as avian influenza or bird flu, is a subtype of the Influenza A virus. H5N1 has pandemic potential due to its expected continued mutation in birds. The dominant strain, HPAI A (H5N1), evolved into the Z genotype, also called Asian lineage HPAI/A/H5N1. This lineage is divided into two antigenic clades. Clade 1 includes human and bird isolates from Vietnam, Thailand, and Cambodia, as well as bird isolates from Laos and Malaysia. Clade 2 viruses, first identified in bird isolates from China, Indonesia, Japan, and South Korea, spread westward to the Middle East, Europe, and Africa.
Description
Hybridoma clones producing this antibody were generated by fusing Sp2/0 myeloma cells with spleen cells isolated from Balb/c mice immunized with purified avian influenza virus type A H5N1.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The antibody is formulated in phosphate-buffered saline (PBS) at a pH of 7.4 and contains 0.1% sodium azide (NaN3) as a preservative.
Shipping Conditions
The antibody is shipped in liquid form with ice packs to maintain a cool temperature during transport.
Storage Procedures
Upon receipt, the antibody should be stored at 4 degrees Celsius. Freezing the antibody is not recommended.
Applications
This antibody is suitable for detecting Influenza A haemagglutinin 1 H5N1 in various immunodetection assays, including direct and indirect enzyme-linked immunosorbent assays (ELISA) and Western blotting.
Purification Method
Chromatography on protein G Sepharose.
Type
Mouse Antibody Monoclonal.
Clone
IA-H5N1.
Immunogen
Influenza A hemagglutinin H5N1.
Ig Subclass
Mouse
IgG2a.
Product Science Overview
Introduction
Influenza A virus is a significant pathogen responsible for seasonal flu epidemics and occasional pandemics. Among the various subtypes, H5N1 is particularly notable due to its high pathogenicity and potential to cause severe respiratory illness in humans and animals. Hemagglutinin (HA) is a glycoprotein found on the surface of the influenza virus and plays a crucial role in the virus’s ability to infect host cells. The H5N1 subtype of hemagglutinin is a key target for both diagnostic and therapeutic interventions. Mouse antibodies against H5N1 hemagglutinin are valuable tools in research and clinical applications.
Preparation Methods
The production of mouse antibodies against H5N1 hemagglutinin typically involves the following steps:
- Immunization: Mice are immunized with purified, recombinant H5N1 hemagglutinin protein. This process involves multiple injections over a period of time to ensure a robust immune response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells. These hybridoma cells are capable of producing monoclonal antibodies.
- Screening and Selection: The hybridoma cells are screened for their ability to produce antibodies that specifically bind to H5N1 hemagglutinin. Positive clones are selected and further cultured.
- Purification: The antibodies produced by the hybridoma cells are purified using techniques such as Protein A affinity chromatography.
Analysis of Chemical Reactions
Mouse antibodies against H5N1 hemagglutinin can be analyzed using various biochemical and immunological techniques:
- Western Blotting: This technique is used to detect the presence of H5N1 hemagglutinin in a sample. The antibodies bind to the hemagglutinin protein, allowing for its visualization on a membrane.
- ELISA (Enzyme-Linked Immunosorbent Assay): ELISA is used to quantify the amount of H5N1 hemagglutinin in a sample. The antibodies are used as capture or detection agents in the assay.
- Immunofluorescence: This technique involves labeling the antibodies with fluorescent dyes to visualize the localization of H5N1 hemagglutinin in cells or tissues.
- Mass Spectrometry: Peptide mapping of the antibody fragments using mass spectrometry can provide detailed information about the antibody’s specificity and structure .
Applications
Mouse antibodies against H5N1 hemagglutinin have several important applications:
- Diagnostics: These antibodies are used in diagnostic assays to detect the presence of H5N1 virus in clinical samples.
- Therapeutics: Monoclonal antibodies can be used as therapeutic agents to neutralize the virus and prevent infection.
- Vaccine Development: Antibodies are used in the development and evaluation of vaccines against H5N1 influenza.
- Research: These antibodies are valuable tools in basic and applied research to study the structure, function, and immunogenicity of H5N1 hemagglutinin .
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