H5N1 Polyclonal Antibody

Influenza-A Hemagglutinin H5N1, Polyclonal Rabbit Antibody
Cat. No.
BT3973
Source
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
90%.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
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Description

Influenza Hemagglutinin protein is an envelope glycoprotein responsible for binding to sialic receptors and influenza viral entry into host cells. The antibody was produced by immunization of rabbits with purified recombinant influenza A H5N1 produced in insect cells using baculovirus expression vector system. The antigen was purified under conditions that preserve the HA proteins biological activity and tertiary structure.

Product Specs

Introduction
Influenza A virus subtype H5N1, commonly known as avian influenza or bird flu, is a subtype of the Influenza A virus. H5N1 has the potential to cause multiple influenza pandemics due to its expected continued mutation in birds. The dominant strain, HPAI A (H5N1), evolved into the Z genotype, also called Asian lineage HPAI/A/H5N1. This lineage is divided into two antigenic clades: Clade 1, encompassing human and bird isolates from Vietnam, Thailand, Cambodia, and bird isolates from Laos and Malaysia; and Clade 2, initially identified in bird isolates from China, Indonesia, Japan, and South Korea before spreading westward to the Middle East, Europe, and Africa.
Description
Influenza Hemagglutinin protein, an envelope glycoprotein, facilitates influenza viral entry into host cells by binding to sialic receptors. This antibody was produced by immunizing rabbits with purified recombinant influenza A H5N1, which was produced in insect cells using a baculovirus expression vector system. The antigen was purified under conditions designed to maintain its biological activity and tertiary structure.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PBS pH 7.0, 0.005% NaN3, and 50% glycerol.
Shipping Conditions
Antibody is shipped in liquid form with ice packs.
Storage Procedures
Store at -20°C; stable for one month at 4°C.
Purity
90%
Applications
Suitable for Western Blotting applications using Influenza A haemagglutinin H1N1 at a concentration of 0.5 µg/ml. ELISA titer to be determined.
Type
Rabbit Antibody Polyclonal.
Clone
IHA-H5N1-PRS.
Immunogen
Recombinant Influenza A/H5N1.

Product Science Overview

Introduction

Influenza A virus is a significant pathogen responsible for seasonal flu epidemics and occasional pandemics. Among its various subtypes, H5N1 is particularly noteworthy due to its high pathogenicity and potential to cause severe respiratory illness in humans and birds. The hemagglutinin (HA) protein on the surface of the virus plays a crucial role in viral entry into host cells. Polyclonal antibodies against this protein, especially those developed in rabbits, are valuable tools in research and diagnostics.

Hemagglutinin (HA) Protein

The HA protein is a glycoprotein found on the surface of the influenza virus. It facilitates viral entry by binding to sialic acid receptors on the surface of host cells, leading to endocytosis of the virus. The HA protein is also a primary target for the host immune response, making it a critical component in vaccine development and serological assays .

H5N1 Subtype

H5N1 is a subtype of the Influenza A virus that has caused several outbreaks in poultry and humans. It is highly pathogenic, with a mortality rate of about 60% in infected humans. The H5N1 virus has undergone several genetic changes, leading to the emergence of different strains. The HA protein of H5N1 is highly variable, which poses challenges for vaccine development and necessitates continuous monitoring and updating of vaccines .

Polyclonal Rabbit Antibodies

Polyclonal antibodies are a mixture of antibodies produced by different B cell clones in the body. They can recognize and bind to multiple epitopes on a single antigen. Polyclonal antibodies against the HA protein of H5N1 are typically produced by immunizing rabbits with the HA antigen. These antibodies are then harvested from the rabbit serum.

Applications

Polyclonal rabbit antibodies against H5N1 HA are used in various scientific applications, including:

  • ELISA (Enzyme-Linked Immunosorbent Assay): Used to detect and quantify the presence of H5N1 HA in samples.
  • Western Blot: Used to identify the HA protein in a mixture of proteins.
  • Immunohistochemistry: Used to detect HA protein in tissue samples.
  • Immunoprecipitation: Used to isolate the HA protein from a mixture of proteins.
  • Immunocytochemistry: Used to detect HA protein in cultured cells .

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