IL 33 Rat, His

Interleukin-33 (IL-33) is a proinflammatory cytokine that plays a crucial role in the immune system. It is part of the IL-1 family and is structurally related to IL-1. IL-33 is known to induce helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1, also known as ST2 .

IL-33 is a 32kDa protein that can regulate gene transcription in producer cells . It binds to the IL1RL-1/ST2 receptor, activating NF-kappa-B and MAP kinases, which in turn induces Th2 cells to produce cytokines such as IL-4, IL-5, and IL-13 . This cytokine is involved in the maturation of Th2 cells and activation of mast cells, basophils, eosinophils, and natural killer cells .

The recombinant IL-33 (Rat, His Tag) is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 179 amino acids (109-264 a.a) with a molecular mass of 19.8kDa . It is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques .

Recombinant IL-33 is used in various laboratory research applications, including studies on immune responses and inflammation. The protein solution is typically formulated with phosphate-buffered saline (pH 7.4) and 10% glycerol . For short-term storage, it is recommended to store the solution at 4°C, while for long-term storage, it should be frozen at -20°C with a carrier protein to avoid multiple freeze-thaw cycles .