IL 33 Mouse

Interleukin-33 Mouse Recombinant
Cat. No.
BT4910
Source
Escherichia Coli.
Synonyms
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, Il-33, Il1f11, 9230117N10Rik, Il33.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity

Greater than 90.0% as determined by analysis by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin33 Mouse recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids and having a molecular mass of 17.7 kDa.

Product Specs

Introduction
Interleukin 33 (IL-33), a 32kDa proinflammatory cytokine, plays a dual role by influencing gene transcription in producer cells and mediating inflammation. Structurally similar to IL-1, IL-33 stimulates helper T cells to release type 2 cytokines. Its action is mediated through the IL1RL-1 receptor (also known as ST2), triggering the activation of NF-kappa-B and MAP kinases. This activation further induces the production of cytokines by Th2 cells in vitro. In vivo, IL-33 elevates IL-4, IL-5, and IL-13 expression, leading to significant pathological changes in mucosal organs. Notably, caspase-1 can cleave IL-33 into a 12kDa N-terminal and an 18kDa C-terminal fragment in vitro.
Description

Recombinant Mouse Interleukin 33, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 159 amino acids. This protein has a molecular weight of 17.7 kDa.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation

The Mouse IL-33 was lyophilized from a sterile, 0.2 micron filtered aqueous solution containing 10mM sodium phosphate, with a pH of 7.5.

Solubility
To reconstitute the lyophilized IL-33, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted with other aqueous solutions as needed.
Stability
Lyophilized IL-33 Recombinant remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, the IL-33 solution should be stored at 4°C for no longer than 2-7 days. For extended storage, freeze the solution below -18°C. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity

The purity is determined to be greater than 90.0% based on SDS-PAGE analysis.

Biological Activity

The ED50, determined by the dose-dependent stimulation of murine D10S cell proliferation, is 0.04ng/ml. This corresponds to a specific activity of 2.5 x 107 units/mg.

Synonyms
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, Il-33, Il1f11, 9230117N10Rik, Il33.
Source
Escherichia Coli.
Amino Acid Sequence

MSIQGTSLLT QSPASLSTYN DQSVSFVLEN GCYVINVDDS GKDQEQDQVL LRYYESPCPA SQSGDGVDGK KLMVNMSPIK DTDIWLHAND KDYSVELQRG DVSPPEQAFF VLHKKSSDFV SFECKNLPGT YIGVKDNQLA LVEEKDESCN NIMFKLSKI.

Product Science Overview

Structure and Expression

IL-33 is initially synthesized as a 32 kDa precursor protein. Upon cellular stress or damage, it is cleaved by caspases to generate a mature 18 kDa form that is biologically active . The mature IL-33 protein contains 158 amino acid residues and has a molecular mass of approximately 17.5 kDa .

Function and Signaling

IL-33 functions as an alarmin, a type of molecule released upon cell damage to alert the immune system. It binds to the ST2 receptor (IL1RL1) on target cells, leading to the activation of NF-κB and MAPK signaling pathways . This activation results in the production of various cytokines and chemokines, contributing to the inflammatory response .

Biological Activity

IL-33 plays a crucial role in Th2-mediated immune responses. It stimulates the production of cytokines such as IL-4, IL-5, and IL-13 in Th2 cells, which are involved in allergic inflammation and defense against parasitic infections . Additionally, IL-33 is involved in the proliferation and activation of group 2 innate lymphoid cells (ILC2s), which further amplifies the Th2 response .

Applications in Research

Recombinant mouse IL-33 is widely used in research to study its role in various biological processes and diseases. It is utilized in cell proliferation assays, where its ability to promote the proliferation of specific cell lines, such as D10.G4.1 mouse helper T cells, is measured . The recombinant protein is also used in studies investigating the mechanisms of allergic inflammation, autoimmune diseases, and other inflammatory conditions .

Storage and Stability

Recombinant mouse IL-33 is typically lyophilized and can be reconstituted in sterile PBS for use in experiments. It is recommended to store the reconstituted protein at -20°C to -70°C to maintain its stability and avoid repeated freeze-thaw cycles .

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