IL 33 Rat

IL-33 is a 32 kDa proinflammatory cytokine that can also regulate gene transcription in producer cells . Structurally, it is related to IL-1 and induces helper T cells to produce type 2 cytokines . The recombinant rat IL-33 is produced in Escherichia coli as a single, non-glycosylated polypeptide chain containing 156 amino acids, with a molecular mass of approximately 17.4 kDa .

IL-33 exerts its effects by binding to the receptor IL1RL-1 (IL1 receptor-like-1), also known as ST2 . This binding activates NF-κB and MAP kinases, leading to the production of cytokines such as IL-4, IL-5, and IL-13 . In vivo, IL-33 induces severe pathological changes in mucosal organs .

The biological activity of IL-33 is significant in various immune responses. It stimulates target cells by binding to the IL-1R/TLR superfamily member ST2, subsequently activating NF-κB and MAPK pathways . This cytokine plays a role in allergic diseases, including allergic rhinitis and chronic rhinosinusitis, and is elevated in conditions like atopic dermatitis .

Recombinant rat IL-33 is used primarily for laboratory research. It is lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, and is recommended to be reconstituted in sterile water . The stability of lyophilized IL-33 is maintained at room temperature for up to three weeks, but for long-term storage, it should be kept below -18°C . Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for future use .

The purity of recombinant rat IL-33 is greater than 95% as determined by SDS-PAGE . It is important to note that this product is intended for laboratory research use only and is not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .