IL33 Mouse, His
Description
IL33 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 superfamily. It is known by several synonyms, including C9orf26, DKFZp586H0523, DVS27, NF-HEV, NFEHEV, and RP11-575C20.2 . IL-33 plays a crucial role in the immune system by inducing helper T cells, mast cells, eosinophils, and basophils to produce type 2 cytokines .
The recombinant mouse IL-33 protein, tagged with a polyhistidine (His) tag, is expressed in human 293 cells (HEK293). The protein consists of amino acids Serine 109 to Isoleucine 266, with a calculated molecular weight of 19.5 kDa . Due to glycosylation, the protein migrates as 20-30 kDa under reducing conditions in SDS-PAGE .
IL-33 mediates its biological effects by interacting with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP). This interaction activates intracellular molecules in the NF-κB and MAP kinase signaling pathways, driving the production of type 2 cytokines such as IL-5 and IL-13 from polarized Th2 cells . In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13, leading to severe pathological changes in mucosal organs .
The recombinant mouse IL-33 protein is lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4, with trehalose added as a protectant before lyophilization . The protein has a purity of greater than 90% as determined by SDS-PAGE . It is recommended to follow the reconstitution protocol provided in the Certificate of Analysis for optimal performance .
For long-term storage, the lyophilized product should be stored at -20°C or lower. It is stable for 12 months in the lyophilized state at -20°C to -70°C and for 3 months under sterile conditions after reconstitution at -70°C . Repeated freeze-thaw cycles should be avoided to maintain protein integrity .
