IL 1 Alpha Rhesus Macaque
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Description
Product Specs
To ensure long-term stability, it is advisable to add a carrier protein (0.1% HSA or BSA) to the solution.
Repeated freezing and thawing cycles should be avoided.
(a) High-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
The ED50, which represents the effective dose required to achieve 50% of the maximum response, was determined through a cell proliferation assay using murine D10S cells. The ED50 is less than 10 pg/ml, indicating a specific activity of greater than 1.0 × 108 IU/mg.
Product Science Overview
Recombinant Rhesus Macaque IL-1α is a single non-glycosylated polypeptide chain containing 159 amino acids . The amino acid sequence of mature IL-1α is conserved across various species, with a conservation rate of 60% to 70% . This recombinant protein is produced in E. coli and is fully biologically active when compared to standard IL-1α .
IL-1α is known for its significant role in inflammation and immune response. It binds to the same receptor as IL-1β, another member of the IL-1 family, and induces a wide range of immune responses . The recombinant form of IL-1α from Rhesus Macaque has been found to be biologically active on murine cell lines, demonstrating its cross-species functionality .
Recombinant IL-1α is used in various research applications, including studies on inflammation, immune response, and cytokine signaling. It is also utilized in cell proliferation assays, where it has shown an ED50 of less than 10 pg/ml, corresponding to a specific activity of greater than 1.0 x 10^8 IU/mg .
The lyophilized preparation of recombinant Rhesus Macaque IL-1α is stable at 2-8°C but should be kept at -20°C for long-term storage . Upon reconstitution, it is most stable at -20 to -80°C and can be stored for one week at 2-8°C . For maximal stability, it is recommended to apportion the reconstituted preparation into working aliquots and store at -20 to -80°C, avoiding repeated freeze/thaw cycles .
