IL 1 alpha Human, His

Interleukin-1 alpha Human Recombinant, His Tag
Cat. No.
BT28946
Source
Escherichia Coli.
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IL-1A Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 159 amino acids fragment (113-271) with an amino-terminal hexahistidine tag and having a total molecular mass of 22.5kDa.
The Interleukin-1 alpha His-tag is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-1 alpha (IL-1 alpha) is a pro-inflammatory cytokine with a wide range of cellular origins, including macrophages, osteoblasts, monocytes, and hepatocytes. Typically found in low concentrations in circulation, IL-1 alpha levels elevate in response to stimuli such as inflammation, infection, or microbial endotoxins. Its biological activities are diverse, and it exerts its effects by binding to specific cell surface receptors.
Description
Recombinant human IL-1A, expressed in E. coli, is a non-glycosylated polypeptide chain containing 159 amino acids (fragment 113-271). It includes an amino-terminal hexahistidine tag and has a molecular mass of 22.5 kDa. The purification of the His-tagged IL-1 alpha is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The His-tagged IL-1 alpha protein is supplied in a buffer of 1x PBS with 50% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it should be stored frozen at -20°C. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 95.0% using the following methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Source
Escherichia Coli.

Product Science Overview

Introduction

Interleukin-1 alpha (IL-1α) is a cytokine belonging to the interleukin 1 family, which plays a crucial role in the regulation of immune responses, inflammation, and hematopoiesis. It is produced by various cell types, including macrophages, monocytes, and epithelial cells . The recombinant form of IL-1α, tagged with a histidine (His) tag, is widely used in research and therapeutic applications due to its ease of purification and enhanced stability.

Structure and Production

IL-1α is a non-glycosylated polypeptide chain consisting of 159 amino acids, with a molecular mass of approximately 22.5 kDa . The His tag, typically composed of six histidine residues, is added to the N-terminus of the protein to facilitate purification using affinity chromatography techniques . The recombinant IL-1α is commonly produced in Escherichia coli (E. coli) expression systems, which allow for high-yield production and cost-effective manufacturing .

Biological Functions

IL-1α is a pleiotropic cytokine involved in various biological processes, including:

  • Immune Response: IL-1α plays a central role in the activation and regulation of immune cells, such as T cells and macrophages .
  • Inflammation: It is a key mediator of the inflammatory response, promoting the production of other pro-inflammatory cytokines and chemokines .
  • Apoptosis: IL-1α can induce apoptosis in certain cell types, contributing to the removal of damaged or infected cells .
  • Hematopoiesis: It is involved in the regulation of hematopoietic stem cells and the production of blood cells .
Applications

Recombinant IL-1α with a His tag is used in various research and therapeutic applications, including:

  • Immunology Research: Studying the role of IL-1α in immune responses and inflammatory diseases.
  • Drug Development: Screening potential therapeutic agents targeting IL-1α or its signaling pathways.
  • Cell Culture: Using IL-1α to stimulate immune cells in vitro for research purposes.
Storage and Stability

Recombinant IL-1α (His tag) should be stored at -20°C to maintain its stability and activity. It is recommended to avoid repeated freeze-thaw cycles to prevent protein degradation .

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THE BIOTEK
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