IL 1 alpha Rat

Recombinant rat IL-1α is typically produced in Escherichia coli (E. coli) and is a non-glycosylated polypeptide chain containing 155 amino acids with a molecular mass of approximately 17,703 Daltons . The recombinant form is often used in research to study its biological activities and interactions.

IL-1α is primarily expressed by monocytes, macrophages, and dendritic cells. It is coded by the IL1A gene located on the q arm of chromosome 2 at position 13 . This cytokine plays a significant role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses .

IL-1α signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1β . Upon binding to its receptors, IL-1α activates various intracellular signaling pathways that lead to the expression of genes involved in inflammation, immune response, and cell proliferation.

Recombinant rat IL-1α is widely used in research to understand its role in various physiological and pathological processes. It is utilized in cell proliferation assays, where its activity is measured using specific cell lines such as D10.G4.1 mouse helper T cells . The recombinant protein is also employed in enzyme-linked immunosorbent assays (ELISAs) to quantify its concentration in biological samples .

Recombinant rat IL-1α is typically lyophilized from a filtered solution in phosphate-buffered saline (PBS) and can be reconstituted in sterile PBS containing at least 0.1% human or bovine serum albumin . It is shipped at ambient temperature and should be stored at -20 to -70°C to maintain its stability. Once reconstituted, it can be stored under sterile conditions at 2 to 8°C for up to one month or at -20 to -70°C for up to three months .