IL 1 alpha Mouse
Interleukin-1 alpha Mouse Recombinant
Cat. No.
BT29033
Source
Escherichia Coli.
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Interleukin-1A Mouse Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 156 amino acids and having a molecular mass of 17.9 kDa.
The IL-1A is purified by proprietary chromatographic techniques.
The IL-1A is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-1 alpha (IL-1α) is a potent pro-inflammatory cytokine that plays a crucial role in the immune response. Produced by various cells, including macrophages and monocytes, IL-1α is released upon stimulation by infection, injury, or other inflammatory signals. Its effects are mediated by binding to specific receptors on target cells, leading to a cascade of events that contribute to inflammation and immune activation.
Description
This product consists of recombinant Interleukin-1A (IL-1A) derived from mice. Produced in E. coli, this non-glycosylated protein is a single polypeptide chain composed of 156 amino acids, with a molecular weight of 17.9 kDa. The purity of IL-1A is achieved through proprietary chromatographic techniques.
Physical Appearance
This product is supplied as a sterile, white powder obtained by lyophilization (freeze-drying).
Formulation
This product was lyophilized from a sterile solution containing 1 mg/ml of the protein in a phosphate-buffered saline (PBS) buffer at pH 7.4.
Solubility
To reconstitute the lyophilized Interleukin-1a, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-1a is stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product desiccated at temperatures below -18°C. Upon reconstitution, IL1A should be stored at 4°C for short-term use (2-7 days). For long-term storage after reconstitution, add a carrier protein (0.1% HSA or BSA) and store below -18°C. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 97.0% as determined by two independent analytical methods:
(a) High-performance liquid chromatography (RP-HPLC)
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
(a) High-performance liquid chromatography (RP-HPLC)
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Biological Activity
The biological activity of this product was assessed by its ability to stimulate the proliferation of murine D10S cells. The ED50, which represents the concentration of IL-1A required to achieve half-maximal stimulation, is determined to be less than 0.002 ng/ml.
Synonyms
Hematopoietin-1, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL-1 alpha,IL1, IL-1A, IL1F1.
Source
Escherichia Coli.
Amino Acid Sequence
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS.
Product Science Overview
Structure and Production
IL-1α is synthesized as a precursor protein (pro-IL-1α) and can be cleaved into smaller mature forms. Both the precursor and the cleaved forms are biologically active and can activate signaling pathways through the membrane receptor IL-1R1 . Unlike its counterpart IL-1β, which is a secreted cytokine, IL-1α is predominantly cell-associated .
Biological Functions
IL-1α has a broad range of biological activities, including:
- Stimulation of thymocyte proliferation by inducing IL-2 release.
- B-cell maturation and proliferation.
- Mitogenic activity similar to fibroblast growth factors (FGF).
- Stimulation of prostaglandin and collagenase release from synovial cells .
Due to its cell-associated nature, IL-1α can act as an “alarmin” that alerts the host to damage or injury when passively leaked from dying cells. It can also enter the nucleus to modulate transcription .
Recombinant IL-1α (Mouse)
Applications and Stability
Recombinant IL-1α is used in various laboratory research applications, including cell proliferation assays and studies on immune responses and inflammation. The product remains stable for up to six months at -70°C or -20°C and for up to one week at 4°C after reconstitution. It is important to avoid repeated freeze-thaw cycles to maintain its biological activity .
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