IL 1 alpha Rat, His

Interleukin-1 alpha (IL-1 alpha) is a cytokine produced by activated macrophages. It plays a crucial role in the inflammatory response, stimulating thymocyte proliferation by inducing interleukin-2 release, B-cell maturation, and fibroblast growth factor activity. IL-1 alpha is also identified as an endogenous pyrogen, stimulating the release of prostaglandin and collagenase from synovial cells .

The recombinant form of rat IL-1 alpha is produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 179 amino acids, with a molecular mass of approximately 20.2 kDa. The recombinant protein is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification through chromatographic techniques .

The recombinant IL-1 alpha is provided as a sterile, filtered, colorless solution. The formulation typically includes phosphate-buffered saline (pH 7.4) and 10% glycerol. The protein exhibits a purity greater than 95.0% as determined by SDS-PAGE .

For short-term storage (2-4 weeks), the recombinant IL-1 alpha should be kept at 4°C. For longer periods, it should be stored frozen at -20°C. To ensure long-term stability, it is recommended to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA). Multiple freeze-thaw cycles should be avoided to maintain protein integrity .

The biological activity of recombinant rat IL-1 alpha is measured using a cell proliferation assay with D10.G4.1 mouse helper T cells. The effective dose (ED50) for this effect is less than or equal to 20 pg/ml .

Recombinant IL-1 alpha is widely used in laboratory research to study its role in the inflammatory response and its effects on various cell types. It is important to note that this product is intended for research use only and is not suitable for use as a drug, agricultural or pesticidal product, food additive, or household chemical .