IL 17 Human, His

Interleukin-17 (IL-17) is a proinflammatory cytokine produced by activated T cells. It plays a crucial role in the immune response by regulating the activities of NF-kappaB and mitogen-activated protein kinases . IL-17 stimulates the expression of other cytokines such as IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO) .

The human recombinant IL-17 with a His tag is produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 132 amino acids, with a molecular weight of approximately 19.62 kDa . The His tag, which is a sequence of histidine residues, is fused to the amino-terminal end of the protein, adding an additional 4.5 kDa to the molecular weight .

The recombinant IL-17 is purified using proprietary chromatographic techniques to achieve a purity greater than 95% as determined by SDS-PAGE . The protein is supplied as a sterile filtered clear solution in 25 mM sodium acetate (pH 4.8) and 50% glycerol . For optimal stability, it should be stored at 4°C if used within 2-4 weeks, or frozen at -20°C for longer periods .

IL-17 is known for its role in promoting inflammation. It can stimulate the production of other proinflammatory cytokines and chemokines, which recruit immune cells to sites of infection or injury . High levels of IL-17 are associated with several chronic inflammatory diseases, including rheumatoid arthritis, psoriasis, and multiple sclerosis .

Recombinant IL-17 with a His tag is widely used in laboratory research to study its role in inflammation and immune response. It is also used in assays to screen for potential therapeutic agents that can modulate IL-17 activity .

ProSpec’s recombinant IL-17 is intended for laboratory research use only and should not be used as drugs, agricultural or pesticidal products, food additives, or household chemicals . Proper safety protocols should be followed when handling this protein to avoid contamination and ensure accurate experimental results .