IL 9 Human
Interleukin-9 Human Recombinant
Cat. No.
BT7314
Source
Escherichia Coli.
Synonyms
P40, HP40, T-cell growth factor p40, IL-9, P40 cytokine.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Interleukin-9 Human Recombinant produced in E.Coli is a single, non-glycosylated single polypeptide chain containing 127 amino acids and having a molecular mass of 14,004 Dalton.
The IL-9 is purified by proprietary chromatographic techniques.
The IL-9 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-9 (IL-9) is a cytokine believed to play a role in regulating hematopoiesis. Studies have shown that it promotes the growth of specific blood cell types, including human mast cells, megakaryoblastic leukemic cells, and murine helper T-cell clones. This glycoprotein, with a molecular weight ranging from 32 to 39 kDa, is produced by T-cells and its corresponding gene is located on human chromosome 5.
Description
Recombinant human Interleukin-9, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 127 amino acids. With a molecular weight of 14,004 Daltons, this purified IL-9 is obtained through proprietary chromatographic techniques.
Physical Appearance
Sterile, white, lyophilized (freeze-dried) powder.
Formulation
The IL-9 protein was lyophilized from a 0.2 µm filtered solution concentrated in phosphate-buffered saline (PBS) at a pH of 7.4.
Solubility
To reconstitute the lyophilized Interleukin-9, it is recommended to dissolve it in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-9 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, IL-9 should be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C is recommended, preferably with the addition of a carrier protein such as 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
The purity of this product exceeds 97.0% as determined by: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) analysis. (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis.
Biological Activity
The ED₅₀, determined by the dose-dependent stimulation of human MO7e cells, is less than 0.2 ng/ml. This corresponds to a specific activity greater than 5.0 x 10⁶ IU/mg.
Synonyms
P40, HP40, T-cell growth factor p40, IL-9, P40 cytokine.
Source
Escherichia Coli.
Amino Acid Sequence
MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI.
Product Science Overview
Discovery and Structure
IL-9 was initially discovered as a T cell growth factor and is best known for its role in promoting the survival and activation of various immune cells, including T cells, mast cells, B cells, and structural cells . The human IL-9 gene is located on chromosome 5 and encodes a protein that is approximately 144 amino acids long .
Production and Secretion
Biological Functions
IL-9 has several important biological functions:
- T Cell Stimulation: IL-9 acts as a growth factor for T cells, promoting their proliferation and survival .
- Mast Cell Activation: It plays a role in the activation and proliferation of mast cells, which are involved in allergic responses .
- B Cell Activation: IL-9 enhances the survival and function of B cells, which are responsible for antibody production .
- Hematopoietic Cell Differentiation: It regulates the differentiation of hematopoietic cells, which are the precursors to various blood cells .
- Tumor Growth: IL-9 has been implicated in the growth of certain types of tumors, including lymphomas and other T cell-mediated cancers .
Recombinant Human IL-9
Recombinant human IL-9 is a synthetic form of the cytokine produced using recombinant DNA technology. It is optimized for use in various research applications, including cell culture, differentiation studies, and functional assays . Recombinant IL-9 is typically expressed in systems such as E. coli or HEK293 cells and is purified to high levels of purity (>95%) with low endotoxin levels .
Applications in Research
Recombinant human IL-9 is widely used in scientific research to study its effects on immune cells and its potential therapeutic applications. Some of the key applications include:
- T Cell and Mast Cell Proliferation Assays: Researchers use recombinant IL-9 to stimulate the proliferation of T cells and mast cells in vitro .
- Hematopoietic Cell Differentiation Studies: IL-9 is used to investigate its role in the differentiation of hematopoietic cells .
- Cancer Research: Studies explore the role of IL-9 in tumor growth and its potential as a therapeutic target in cancer treatment .
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