IL 9 Rat

Interleukin-9 Rat Recombinant
Cat. No.
BT7576
Source
Escherichia Coli.
Synonyms
Interleukin 9, Protein Il9, Il9.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-9 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of 14.3kDa.
The IL-9 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-9 (IL-9) is believed to play a role in regulating hematopoiesis, the formation of blood cellular components. Studies have demonstrated its ability to promote the growth of various cells, including human mast cells, megakaryoblastic leukemic cells, and murine helper T-cell clones. This glycoprotein, with a molecular weight ranging from 32 to 39 kDa, originates from T-cells and is located on chromosome 5 in humans.
Description
Recombinant Rat Interleukin-9, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 127 amino acids, resulting in a molecular weight of 14.3 kDa. The purification process of IL-9 involves proprietary chromatographic techniques.
Physical Appearance
White, lyophilized (freeze-dried) powder, sterile and filtered.
Formulation
The IL-9 protein was lyophilized from a 0.2 μm filtered solution concentrated in PBS at a pH of 7.4.
Solubility
For reconstitution of the lyophilized Interleukin-9, it is advised to use sterile 18 MΩ-cm H2O at a concentration not less than 100 μg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-9, though stable at room temperature for a period of 3 weeks, is best stored in dry conditions below -18°C. After reconstitution, IL-9 should be stored at 4°C for 2-7 days. For extended storage, freezing the solution below -18°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 97.0% through the following analyses: (a) Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and (b) Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The ED50, determined by a cell proliferation assay using murine TS1 cells, is less than 10 ng/ml. This corresponds to a specific activity exceeding 1.0 × 105 IU/mg.
Synonyms
Interleukin 9, Protein Il9, Il9.
Source
Escherichia Coli.
Amino Acid Sequence
MQRCSTSWGI QHTSYLIENL KDDPSSKCSC SANVTSCLCL PIPSDDCTTP CFQEGMSQVT NATQQSKFSP FFFRVKRIVE TLKSNKCQFF SCEKPCNQTT AGNTVSFLKS LLKTFQKTEV QVQRSRA.

Product Science Overview

Structure and Function

IL-9 is a member of the type I cytokine receptor family. The IL-9 receptor (IL-9R) is a protein that specifically mediates the biological effects of IL-9. The receptor is composed of two subunits: the IL-9R alpha subunit and the common gamma chain (γc). The binding of IL-9 to its receptor leads to the activation of various signaling pathways, including the JAK/STAT pathway, which is crucial for transmitting signals from the cell surface to the nucleus .

Recombinant IL-9

Recombinant IL-9 refers to IL-9 that is produced through recombinant DNA technology. This involves inserting the gene encoding IL-9 into a host cell, such as bacteria or yeast, which then produces the cytokine. Recombinant IL-9 is used in research to study its effects on various cell types and to understand its role in diseases such as asthma and allergies .

Applications in Research

Recombinant rat IL-9 is particularly useful in research involving rodent models. It is used to study the effects of IL-9 on rat immune cells and to investigate the potential therapeutic applications of IL-9 in treating diseases. For example, recombinant rat IL-9 can be used to study its role in mast cell maturation and the enhancement of IgE production by B cells .

Stability and Storage

Recombinant proteins, including IL-9, are typically provided as lyophilized powders. They are stable for up to twelve months when stored at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can degrade the protein .

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