IL 9 Mouse

Interleukin-9 Mouse Recombinant
Cat. No.
BT7478
Source
Escherichia Coli.
Synonyms
P40, HP40, T-cell growth factor p40, IL-9, P40 cytokine.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 96.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-9 Mouse Recombinant produced in E.Coli is a single, non-glycosylated single polypeptide chain containing 127 amino acids and having a molecular mass of 14.3kDa.
The IL-9 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-9 (IL-9) is believed to play a role in regulating hematopoiesis. Studies have shown its ability to stimulate the growth of various cell types, including human mast cells, megakaryoblastic leukemic cells, and murine helper T-cell clones. This glycoprotein, with a molecular weight ranging from 32 to 39 kDa, originates from T-cells and is located on human chromosome 5.
Description
Recombinant Mouse Interleukin-9, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 127 amino acids. With a molecular weight of 14.3 kDa, it is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 1 mg/ml solution in 10 mM Na2PO4, pH 7.5.
Solubility
Reconstitute the lyophilized Interleukin-9 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-9 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, store IL-9 at 4°C for 2-7 days. For long-term storage, freeze at -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 96.0% as determined by SDS-PAGE.
Biological Activity
The ED50, determined by the dose-dependent stimulation of human MO7e cells, is less than 0.5 ng/ml. This corresponds to a Specific Activity of 2,000,000 IU/mg.
Protein Content
Protein content was quantified using two independent methods: 1) UV spectroscopy at 280 nm, employing an absorbance value of 0.6 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is determined using the PC GENE computer analysis program for protein sequences (IntelliGenetics). 2) Analysis by RP-HPLC, utilizing a standard solution of IL-9 as a Reference Standard.
Synonyms
P40, HP40, T-cell growth factor p40, IL-9, P40 cytokine.
Source
Escherichia Coli.
Amino Acid Sequence
MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP.

Product Science Overview

Structure and Production

Recombinant mouse IL-9 is typically produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain. It consists of 127 amino acids and has a molecular mass of approximately 14.3 kDa . The protein includes 10 cysteine residues, which are fully conserved between the human and murine (mouse) proteins .

Biological Activity

IL-9 has several biological activities, primarily related to its role as an immunoregulatory cytokine. It is known to:

  • Stimulate the proliferation of various cell types, including T cells, mast cells, and hematopoietic progenitor cells .
  • Enhance the production of other cytokines, such as IL-6 and IL-10 .
  • Promote the survival and function of mast cells, which are involved in allergic responses .
  • Influence the differentiation and function of T-helper cells, particularly Th2 cells .

The biological activity of recombinant mouse IL-9 is often measured by its ability to stimulate the proliferation of specific cell lines, such as MC/9 cells or human MO7e cells .

Applications

Recombinant mouse IL-9 is used in various research applications, including:

  • Immunology Studies: To investigate the role of IL-9 in immune responses and its potential therapeutic applications in immune-related diseases .
  • Allergy Research: To study the involvement of IL-9 in allergic reactions and its potential as a target for allergy treatments .
  • Hematopoiesis Research: To explore the effects of IL-9 on the formation and differentiation of blood cells .
Storage and Stability

Recombinant mouse IL-9 is typically supplied as a lyophilized (freeze-dried) powder. For long-term storage, it should be kept desiccated at temperatures below -18°C. Upon reconstitution, it is stable for up to one week at 2°C to 8°C. For extended storage, it is recommended to add a carrier protein, such as bovine serum albumin (BSA), and store at -20°C to prevent freeze-thaw cycles .

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