IL-9 Human, Sf9 Active
Interleukin 9, Protein Il9, Il9, HP40, Cytokine P40, T-cell growth factor P40
Greater than 85.0% as determined by SDS-PAGE.
Description
IL-9 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 132 amino acids (19-144 aa) and having a molecular mass of 14.9kDa.
IL-9 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-9 (IL-9) is a protein that plays a role in regulating hematopoiesis. It promotes the growth of certain blood cells, including megakaryoblastic leukemic cells. IL-9 is produced by various immune cells, such as mast cells, Treg cells, NKT cells, Th17 cells, Th2 cells, ILC2 cells, and primarily by Th9 cells, a subset of CD4+ T cells.
Recombinant human IL-9, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. It consists of 132 amino acids (residues 19-144), with a molecular weight of 14.9 kDa. The IL-9 protein includes a 6-amino acid Histidine tag fused at the C-terminus and is purified using proprietary chromatographic techniques.
The IL-9 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. For longer periods, it is recommended to store the product frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freezing and thawing cycles.
The purity of the IL-9 protein is greater than 85% as determined by SDS-PAGE analysis.
The biological activity of IL-9 is assessed through a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 value, representing the effective concentration for half-maximal response, is less than or equal to 0.3 ng/ml.
Interleukin 9, Protein Il9, Il9, HP40, Cytokine P40, T-cell growth factor P40
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Product Science Overview
Human Recombinant IL-9 is produced using Sf9 Baculovirus cells. This method involves using insect cells (Sf9) infected with a baculovirus that carries the gene for human IL-9. The resulting protein is a single, glycosylated polypeptide chain containing 132 amino acids, with a molecular mass of approximately 14.9 kDa . The recombinant IL-9 is fused to a 6 amino acid His tag at the C-terminus, which aids in its purification through chromatographic techniques .
The IL-9 solution is typically formulated with 10% glycerol and phosphate-buffered saline (pH 7.4) to maintain its stability . For short-term storage, it can be kept at 4°C if used within 2-4 weeks. For longer periods, it should be stored frozen at -20°C, with the addition of a carrier protein (0.1% HSA or BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s activity.
