IL5 Mouse, HEK
HEK293 Cells.
interleukin 5, Il, Il-5, B-cell growth factor II, EDF, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, TRFB cell differentiation factor I, T-cell replacing factor, TRF, B-cell differentiation factor I, IL5
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL5 Mouse Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 122 amino acids (21-133 a.a) and having a molecular mass of 14.2 kDa.
IL5 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin-5 (IL-5) is a cytokine crucial for the growth and specialization of B cells and eosinophils. It plays a vital role in the production, maturation, and activation of eosinophils, which are involved in immune responses. Elevated IL-5 levels are linked to conditions like asthma and hypereosinophilic syndromes. IL-5 signals through a heterodimeric receptor, sharing its beta subunit with the receptors for IL-3 and GM-CSF. Notably, the genes for IL-5, IL-4, IL-13, and CSF2 are clustered on chromosome 5 and are co-regulated by extensive regulatory elements spanning a large region of chromosome 5q31.
Recombinant Mouse IL-5, produced in HEK293 cells, is a single-chain glycoprotein. It consists of 122 amino acids (specifically, amino acids 21 to 133), resulting in a molecular weight of 14.2 kDa. The IL-5 is engineered with a 6-amino acid His tag at the C-terminus to facilitate purification, which is carried out using specialized chromatographic methods.
A clear, colorless solution that has been sterilized by filtration.
The IL-5 is provided in a solution with a concentration of 0.25 mg/ml. The solution is buffered with phosphate-buffered saline at a pH of 7.4 and supplemented with 10% glycerol for stability.
For short-term storage (up to 4 weeks), the IL-5 should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to freeze the IL-5 at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (at a concentration of 0.1%) is advised. Repeated freezing and thawing of the IL-5 should be minimized to prevent degradation.
The purity of the IL-5 is determined using SDS-PAGE analysis and is guaranteed to be greater than 90%.
The biological activity of the IL-5 is evaluated using a cell proliferation assay. This assay employs TF-1 human erythroleukemic cells, and the ED50 (the concentration at which 50% of the maximum response is observed) is typically less than or equal to 3 ng/ml.
interleukin 5, Il, Il-5, B-cell growth factor II, EDF, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, TRFB cell differentiation factor I, T-cell replacing factor, TRF, B-cell differentiation factor I, IL5
HEK293 Cells.
DGSMEIPMST VVKETLTQLS AHRALLTSNE TMRLPVPTHK NHQLCIGEIF QGLDILKNQT VRGGTVEMLF QNLSLIKKYI DRQKEKCGEE RRRTRQFLDY LQEFLGVMST EWAMEGHHHH HH
Product Science Overview
IL-5 exerts its effects by binding to the interleukin-5 receptor (IL-5R), a heterodimer composed of an alpha and a beta subunit . The beta subunit is shared with the receptors for interleukin-3 (IL-3) and colony-stimulating factor 2 (CSF2/GM-CSF) . This binding activates signaling pathways that lead to the proliferation and differentiation of target cells.
