IL3 Mouse, sf9

Interleukin-3 (Mouse Recombinant, sf9) is produced in Sf9 Baculovirus cells. The recombinant IL-3 is a single, glycosylated polypeptide chain containing 148 amino acids (27-166 a.a.) and has a molecular mass of 16.7 kDa. Under reducing conditions, it migrates at 18-28 kDa on SDS-PAGE . The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

The IL-3 protein solution is a sterile, filtered, colorless solution. It is formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol. The protein solution has a concentration of 0.25 mg/ml .

For short-term storage (2-4 weeks), the IL-3 protein should be kept at 4°C. For longer periods, it should be stored frozen at -20°C. To ensure long-term stability, it is recommended to add a carrier protein (0.1% HSA or BSA) and avoid multiple freeze-thaw cycles .

The purity of the IL-3 protein is greater than 90.0%, as determined by SDS-PAGE . Its biological activity is measured in a cell proliferation assay using TF-1 human erythroleukemic cells, with an ED50 of ≤ 0.1 ng/ml .

The amino acid sequence of Interleukin-3 (Mouse Recombinant, sf9) is as follows:

ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG 
EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT 
SRPPQPASGS VSPNRGTVEC LEHHHHHH