IL 3 Mouse
Interleukin-3 Mouse Recombinant
Cat. No.
BT4109
Source
Escherichia Coli.
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton.
The IL-3 is purified by proprietary chromatographic techniques.
The IL-3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Interleukin-3 (IL-3) is a cytokine primarily produced by activated T cells that plays a crucial role in hematopoiesis. It acts through specific cell surface receptors, stimulating the proliferation, differentiation, and survival of various hematopoietic cell lines. Additionally, IL-3 influences the functional activity of mast cells, eosinophils, megakaryocytes, and basophils.
Description
Recombinant mouse Interleukin-3, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 135 amino acids, with a molecular weight of 15.1 kDa. It is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, white, lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 1 mg/mL solution in water without any additives.
Solubility
Reconstitute the lyophilized Interleukin-3 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/mL. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Interleukin-3 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. After reconstitution, store IL-3 at 4°C for 2-7 days. For long-term storage, freeze at -18°C after adding a carrier protein like 0.1% HSA or BSA. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0% as determined by: (a) Size-exclusion high-performance liquid chromatography (SEC-HPLC) and (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Biological Activity
The half-maximal effective concentration (ED50), determined by the dose-dependent stimulation of murine M-NFS-60 cells, is less than 0.05 ng/mL, corresponding to a specific activity of 20,000,000 IU/mg.
Protein Content
Protein quantification was conducted using two independent methods: (1) UV spectroscopy at 280 nm with an absorbance value of 0.154 as the extinction coefficient for a 0.1% (1 mg/mL) solution, calculated using the PC GENE computer analysis program (IntelliGenetics); and (2) Reverse-phase high-performance liquid chromatography (RP-HPLC), using a standard solution of IL-3 as a reference standard.
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Source
Escherichia Coli.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.
Product Science Overview
Structure and Receptor Interaction
IL-3 exerts its biological effects through interaction with a specific cell surface receptor complex. The mouse IL-3 receptor consists of a unique alpha-subunit (CD123) and two beta subunits: one specific for IL-3 (βIL-3) and the other shared with the receptors for IL-5 and GM-CSF (beta common chain, βc or CD131) . Binding of IL-3 to its receptor activates several intracellular signaling pathways, including JAK/STAT, MAPK, and PI3K .
Biological Functions
IL-3 is involved in various physiological processes, including:
- Hematopoiesis: IL-3 promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells .
- Inflammation and Allergies: IL-3 plays a role in inflammation and allergic responses by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by mast cells, basophils, and eosinophils .
- Cell Differentiation: IL-3 supports the differentiation of hematopoietic progenitor cells into various mature blood cell lineages .
Recombinant IL-3 Production
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