IL 3 Human, His

Interleukin-3 Human Recombinant, His Tag
Cat. No.
BT3896
Source
Escherichia Coli.
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag.
The IL3 His is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin-3 (IL-3) is a cytokine with diverse functions, primarily produced by activated T cells. It is believed to interact with specific cell surface receptors, thereby promoting the proliferation, differentiation, and survival of hematopoietic cell lines. Additionally, IL-3 has been found to influence the functional activity of various other cell types, such as mast cells, eosinophils, megakaryocytes, and basophils.
Description
Recombinant human Interleukin-3, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 154 amino acids, encompassing a fragment from position 20 to 152, and has a total molecular mass of 17.3 kDa. The protein includes a 20-amino acid N-terminal His tag and is purified using proprietary chromatographic methods.
Physical Appearance
A clear solution, sterilized by filtration.
Formulation
Interleukin-3 His (1 mg/ml) is supplied in a buffer solution containing 20 mM Tris-HCl (pH 8.0), 0.2 mM PMSF, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of the product exceeds 90.0%, as determined by SDS-PAGE analysis.
Biological Activity
The ED50, representing the effective concentration for inducing a 50% response, is less than 0.53 ng/ml. This value was determined using a cell proliferation assay with TF1 human erythroleukemic cells.
Synonyms
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF.

Product Science Overview

Introduction

Interleukin-3 (IL-3) is a potent growth-promoting cytokine that plays a crucial role in the immune system. It belongs to the family of interleukins, which are produced by a variety of body cells and are essential for the proper functioning of the immune system. IL-3 is primarily synthesized by activated T cells, monocytes, macrophages, and endothelial cells .

Structure and Function

IL-3 is a pleiotropic factor, meaning it has multiple effects on different types of cells. It stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage-committed progenitors . This cytokine is involved in the growth and differentiation of erythroid, granulocyte-macrophage, megakaryocyte, and mast cell lineages . Additionally, IL-3 enhances the phagocytic activity of monocytes and eosinophils, mediates antibody-dependent cellular cytotoxicity (ADCC), and stimulates the production of tumor necrosis factor (TNF) and macrophage colony-stimulating factor (M-CSF) .

Recombinant Human IL-3 (His Tag)

Recombinant human IL-3 (His Tag) is a form of IL-3 that has been genetically engineered and expressed in host cells, such as E. coli or HEK293 cells, with a histidine (His) tag at the C-terminus . The His tag facilitates the purification of the recombinant protein using affinity chromatography techniques. This recombinant form of IL-3 retains its biological activity and can be used for various research and therapeutic applications.

Preparation Methods

The preparation of recombinant human IL-3 (His Tag) involves several steps:

  1. Gene Cloning: The gene encoding human IL-3 is cloned into an expression vector that includes a sequence for the His tag.
  2. Transformation: The expression vector is introduced into host cells, such as E. coli or HEK293 cells, through a process called transformation.
  3. Protein Expression: The host cells are cultured under conditions that induce the expression of the recombinant IL-3 protein.
  4. Purification: The recombinant IL-3 protein is purified using affinity chromatography, which exploits the His tag to selectively bind the protein to a nickel or cobalt resin.
  5. Characterization: The purified protein is characterized using techniques such as SDS-PAGE, HPLC, and mass spectrometry to confirm its purity and molecular weight .
Applications

Recombinant human IL-3 (His Tag) has several applications in research and medicine:

  • Hematopoietic Research: IL-3 is used to study the proliferation and differentiation of hematopoietic stem cells and progenitor cells.
  • Immunology: IL-3 is used to investigate its role in immune responses and its potential therapeutic applications in immune-related disorders.
  • Cell Culture: IL-3 is used as a supplement in cell culture media to support the growth of certain cell lines, such as TF-1 human erythroleukemic cells .
  • Drug Development: IL-3 is used in the development of new drugs and therapies for conditions such as hematopoietic failure and immune deficiencies .

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