The recombinant canine IL-3 protein is a single non-glycosylated polypeptide chain containing 120 amino acids . The amino acid sequence of this protein is as follows:
RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP
This sequence corresponds to the IL-3 protein derived from E. coli .
IL-3 is a potent growth-promoting cytokine capable of supporting the proliferation of a broad range of hematopoietic cell types . It is involved in various cell activities such as cell growth, differentiation, and apoptosis . The recombinant canine IL-3 protein is fully biologically active when compared to the standard. The ED50, as determined by a cell proliferation assay using human TF-1 cells, is less than 0.2 ng/ml, corresponding to a specific activity of greater than 5.0 x 10^6 IU/mg .
IL-3 plays a significant role in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood: granulocytes and monocytes-macrophages . This makes it a critical factor in the immune response and in the development of various therapeutic applications.
The lyophilized preparation of recombinant canine IL-3 protein is stable for 12 months from the date of receipt when stored at -20 to -70 degrees Celsius, preferably desiccated . Upon reconstitution, the preparation can be stored for one month at 2-8 degrees Celsius under sterile conditions and for three months at -20 to -70 degrees Celsius . It is recommended to use a manual defrost freezer and avoid repeated freeze-thaw cycles .