IL 3 Human

IL-3 is primarily involved in the regulation of blood cell production. It induces the proliferation and differentiation of both early pluripotent stem cells and committed progenitors . This cytokine has several specific effects, including the regeneration of platelets and potentially aiding in early antibody isotype switching . IL-3 works in conjunction with other β common chain cytokines like GM-CSF and IL-5 to regulate the inflammatory response, helping to clear pathogens by altering the abundance of various cell populations .

The IL-3 receptor is a heterodimer consisting of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5 . Binding of IL-3 to its receptor induces the activation of Jak2, phosphorylation of multiple Stats, and the PI3K/Akt pathway . These signaling pathways are crucial for the cytokine’s role in immune response and inflammation.

Recombinant human IL-3 is produced using E. coli expression systems and is supplied in a lyophilized form . This recombinant form is used in various research and clinical applications to study its effects and potential therapeutic uses. The bioactivity of recombinant IL-3 is measured using human TF-1 erythroleukemic cells, with an ED50 between 0.05-0.3 ng/mL .

IL-3 has been studied for its potential role in treating various conditions, including its involvement in the development of airway inflammation associated with asthma . It is also being explored for its role in the central nervous system, acting as a molecular messenger between the central nervous and peripheral immune systems .