IL 3 Rat

Recombinant rat IL-3 is typically produced in Escherichia coli (E. coli) and purified through sequential chromatography . The recombinant form is a single, non-glycosylated polypeptide chain containing 144 amino acids and has a molecular mass of approximately 16.3 kDa . The amino acid sequence of rat IL-3 includes several key regions that are essential for its biological activity .

IL-3 is known for its ability to stimulate the proliferation and differentiation of various hematopoietic cell types. It exerts its effects by binding to specific receptors on the surface of target cells, leading to the activation of intracellular signaling pathways that promote cell growth and survival . The biological activity of recombinant rat IL-3 is often measured using a cell proliferation assay with NFS-60 mouse myelogenous leukemia lymphoblast cells .

Recombinant rat IL-3 is widely used in research to study its effects on hematopoietic cells and to explore its potential therapeutic applications. It is commonly used in cell culture experiments to promote the growth and differentiation of hematopoietic stem cells and progenitor cells . Additionally, IL-3 is used as a standard in enzyme-linked immunosorbent assays (ELISAs) and other immunoassays to quantify the presence of IL-3 in biological samples .

Recombinant rat IL-3 is typically supplied as a lyophilized powder and should be reconstituted in sterile, distilled water or phosphate-buffered saline (PBS) to regain full activity . It is recommended to store the reconstituted protein at -20°C or lower to maintain its stability and avoid repeated freeze-thaw cycles . The lyophilized form can be stored at 2°C to 8°C, preferably desiccated .