IL2RA Human, sf9
Interleukin 2 Receptor Subunit Alpha, Interleukin 2 Receptor, Alpha, IL-2 Receptor Subunit Alpha, IL-2R Subunit Alpha, TAC Antigen, P55, Insulin-Dependent Diabetes Mellitus 10, Interleukin-2 Receptor Subunit Alpha, CD25 Antigen, IL-2-RA, IDDM10, IL2-RA, IMD41, TCGFR, CD25, IL2R.
Greater than 90.0% as determined by SDS-PAGE.
Description
IL2RA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 461 amino acids (22-240 a.a.) and having a molecular mass of 52.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
IL2RA is expressed with a 239 amino acid hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Interleukin 2 Receptor Subunit Alpha, Interleukin 2 Receptor, Alpha, IL-2 Receptor Subunit Alpha, IL-2R Subunit Alpha, TAC Antigen, P55, Insulin-Dependent Diabetes Mellitus 10, Interleukin-2 Receptor Subunit Alpha, CD25 Antigen, IL-2-RA, IDDM10, IL2-RA, IMD41, TCGFR, CD25, IL2R.
ADPELCDDDP PEIPHATFKA MAYKEGTMLN CECKRGFRRI KSGSLYMLCT GNSSHSSWDN QCQCTSSATR NTTKQVTPQP EEQKERKTTE MQSPMQPVDQ ASLPGHCREP PPWENEATER IYHFVVGQMV YYQCVQGYRA LHRGPAESVC KMTHGKTRWT QPQLICTGEM ETSQFPGEEK PQASPEGRPE SETSCLVTTT DFQIQTEMAA TMETSIFTTE YQLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
Interleukin-2 Receptor Alpha (IL-2RA), also known as CD25, is a protein that plays a crucial role in the immune system. It is one of the three subunits that form the high-affinity interleukin-2 (IL-2) receptor complex, which also includes the beta (IL-2RB) and gamma (IL-2RG) chains . This receptor is essential for the regulation of immune responses, particularly in the activation and proliferation of T cells.
IL-2RA is a transmembrane protein that is primarily expressed on the surface of activated T cells, regulatory T cells (Tregs), and some B cells . The receptor binds to IL-2, a cytokine that promotes the growth, differentiation, and survival of immune cells. The binding of IL-2 to its receptor triggers a cascade of intracellular signaling pathways that lead to T cell proliferation and differentiation .
Recombinant IL-2RA can be produced using various expression systems, including Escherichia coli (E. coli) and insect cells (Sf9). The recombinant protein is often tagged with a histidine tag to facilitate purification. The production in Sf9 cells involves the use of baculovirus expression vectors, which allow for high-level expression of the protein .
Elevated levels of soluble IL-2RA (sIL-2RA) in the serum are associated with various autoimmune diseases, leukemias, and lymphomas . The measurement of sIL-2RA levels can be used as a biomarker for disease activity and prognosis in these conditions. Additionally, IL-2RA is a target for therapeutic interventions in diseases such as multiple sclerosis and certain types of cancer .
