sIL 2R Human
Greater than 95.0% as determined by SDS-PAGE.
Description
The IL-2R is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system by promoting the growth, differentiation, and survival of certain immune cells, including T cells and B cells. The Interleukin-2 receptor (IL-2R) is a heterotrimeric protein complex composed of three subunits: alpha (IL-2Rα or CD25), beta (IL-2Rβ), and gamma (IL-2Rγ). The alpha subunit, also known as CD25, is particularly important for the high-affinity binding of IL-2.
The soluble form of the IL-2 receptor alpha (sIL-2Rα) is a truncated version of the membrane-bound receptor that is released into the bloodstream. This soluble receptor can bind to IL-2, modulating its availability and activity in the immune system. Elevated levels of sIL-2Rα are often associated with various autoimmune diseases, infections, and certain types of cancer, making it a valuable biomarker for these conditions.
Human recombinant sIL-2Rα is a laboratory-produced version of the soluble IL-2 receptor alpha. It is typically produced using recombinant DNA technology, where the gene encoding the human IL-2Rα is inserted into an expression system, such as Escherichia coli (E. coli) or Chinese Hamster Ovary (CHO) cells. The recombinant protein is then purified for use in research and clinical applications.
The production of human recombinant sIL-2Rα involves several steps:
- Gene Cloning: The gene encoding IL-2Rα is cloned into a suitable expression vector.
- Expression: The vector is introduced into an expression system (e.g., E. coli or CHO cells), where the protein is produced.
- Purification: The recombinant protein is purified using chromatographic techniques to achieve high purity levels (typically ≥ 95% by SDS-PAGE) and low endotoxin levels (<1 EU/µg).
Human recombinant sIL-2Rα has several important applications:
- Research: It is used in various immunological assays to study the role of IL-2 and its receptor in the immune system.
- Clinical Diagnostics: Elevated levels of sIL-2Rα in the blood can be used as a biomarker for diagnosing and monitoring autoimmune diseases, infections, and cancers.
- Therapeutics: Recombinant sIL-2Rα can be used in therapeutic applications to modulate the immune response, particularly in conditions where IL-2 activity needs to be regulated.
